Identification of a novel 65-kDa cell surface receptor common to pancreatic polypeptide, neuropeptide Y and peptide YY

Koji Nata, Hideto Yonekura, Hiroshi Yamamoto, Hiroshi Okamoto

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

By affinity cross-linking and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, we identified a novel cell surface receptor on intact rat cells, which bound, with similar dissociation constants, pancreatic polypeptide (PP), neuropeptide Y (NPY) and peptide YY (PYY), the members of the PP family. The receptor was detected on pancreatic islet and acinar cells, hepatocytes and epithelial cells of the stomach, duodenum and small intestine. Its molecular weight was estimated to be 65,000, and the cross-linking of [125I]labeled ligands was inhibited by an excess of unlabeled PP, NPY or PYY. The results suggest that the 65-kDa molecule is a common receptor for PP family peptides.

Original languageEnglish
Pages (from-to)330-335
Number of pages6
JournalBiochemical and biophysical research communications
Volume171
Issue number1
DOIs
Publication statusPublished - 1990 Aug 31

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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