Identification of a cell line producing high levels of TSLP: Advantages for screening of anti-allergic drugs

Ryosuke Segawa, Saori Yamashita, Natsumi Mizuno, Mika Shiraki, Takahiro Hatayama, Nozomi Satou, Masahiro Hiratsuka, Michihiro Hide, Noriyasu Hirasawa

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Thymic stromal lymphopoietin (TSLP) plays critical roles in the induction and exacerbation of allergic diseases. These findings suggest that an inhibitor of TSLP production may be a novel drug for allergic diseases. However, conducting high-throughput screening of such compounds is difficult because there is currently no appropriate in vitro system. In the present study, we demonstrated that the mouse keratinocyte cell line KCMH-1 produced higher amounts of TSLP than the mouse keratinocyte cell line PAM-212, human keratinocyte cell line HaCaT, or bronchial cell line BEAS-2B. A reporter gene assay revealed that transcriptional activity of the TSLP gene was also markedly higher in KCMH-1 than in PAM212 cells. Both dexamethasone and the retinoid X receptor agonist HX600 inhibited the production of TSLP in KCMH-1 cells, which indicated that its production could be pharmacologically regulated. Moreover, the biological activity of TSLP released from KCMH-1 cells in the medium was endorsed by the induction of OX40L expression in bone marrow-derived dendritic cells. These results indicate that KCMH-1 can be utilized in high-throughput screening of inhibitors of TSLP production and also as a source of native TSLP.

Original languageEnglish
Pages (from-to)9-14
Number of pages6
JournalJournal of Immunological Methods
Issue number1-2
Publication statusPublished - 2014


  • Dexamethasone
  • Keratinocyte
  • OX40L
  • Thymic stromal lymphopoietin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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