Abstract
We describe a cardiac muscle isoform of the voltage-dependent calcium channel α1 subunit, which corresponds to the rabbit ortholog of α1C-a (Cav1.2a). We also cloned smooth muscle isoforms α1C-b (Cav1.2b) and α1C-d (Cav1.2d). Differences among these three isoforms lie within the N-terminal region (exon 1A or 1B), the sixth transmembrane segment of domain I (exon 8A or 8B), and the use of exon 10, which forms the intracellular loop between transmembrane domains I and II. Two-hybrid analysis revealed interactions among the three α1 isoforms and β subunits. In vitro overlay and immunoprecipitation analyses revealed preferential binding between α1C-a and β2, which is also expressed at a high level in the heart.
Original language | English |
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Pages (from-to) | 115-125 |
Number of pages | 11 |
Journal | Journal of Molecular and Cellular Cardiology |
Volume | 41 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2006 Jul |
Externally published | Yes |
Keywords
- Cardiac muscle
- Overlay
- Voltage-dependent calcium channel
- cDNA
- α1 subunit
- β subunit
ASJC Scopus subject areas
- Molecular Biology
- Cardiology and Cardiovascular Medicine