Identification of a 120-kDa protein associated with aromatic hydrocarbon receptor nuclear translocator

Anwar Hossain, Hideaki Kikuchi, Shuntaro Ikawa, Ikuko Sagami, Minro Watanabe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aromatic hydrocarbon receptor nuclear translocator (ARNT) is a basic helix-loop-helix-PAS protein which forms a heterodimer with aromatic hydrocarbon receptor (AHR), this heterodimer mediating the signal transduction in response to the various xenobiotics such as 2,3,7,8-tetrachlorodibenzo-p-dioxin and directly interacting with target genes by binding to xenobiotic responsive elements. An anti-ARNT antibody was raised in rabbits against the bacterially expressed ARNT of amino acids 21-328 from the N-terminal. Using this antibody, besides ARNT itself, we detected at least one protein, 120 kDa, in the immunoprecipitate of anti-ARNT antibodies in HepG2 cells as well as in Hepa-1 cells. However, this protein is not present in the immunoprecipitate of the anti-AHR antisera nor in that of the preimmune sera of the rabbits used for the immunization.

Original languageEnglish
Pages (from-to)144-150
Number of pages7
JournalBiochemical and biophysical research communications
Volume212
Issue number1
DOIs
Publication statusPublished - 1995 Jul 6

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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