In human endometrial cancer, we have previously identified a 790-kb region of common allelic loss in chromosome bands 10q25-q26, flanked by D10S587 and D10S1723. We constructed a contig covering the entire deleted region using YACs, PACs, and BACs. Five overlapping cosmid clones derived from YAC clones completely covered the entire deleted region: its size was estimated to be no larger than 200 kb. We further performed two-color fluorescence in situ hybridization (FISH) analysis to confirm the deletion and narrowed down the deleted region to 100 kb or less; it was covered by three overlapping cosmid clones that were included in one BAC clone. Restriction endonuclease mapping identified a region in which Notl, Sail, Sinal, and Xhol were clustered, suggesting the possible existence of a CpG island.
|Number of pages||4|
|Journal||Genes Chromosomes and Cancer|
|Publication status||Published - 1998 Sep|
ASJC Scopus subject areas
- Cancer Research