Identification of a 100-kb region of common allelic loss on chromosome bands 10q25-q26 in human endometrial cancer

Hiromitsu Yamakawa, Satoru Nagase, Michihiro Yuki, Hiromi O. Shiwaku, Toru Furukawa, Kousuke Yoshinaga, Eiichi Soeda, Masato Hoshi, Yutaka Hayashi, Shinji Sato, Akira Yajima, Akira Horii

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In human endometrial cancer, we have previously identified a 790-kb region of common allelic loss in chromosome bands 10q25-q26, flanked by D10S587 and D10S1723. We constructed a contig covering the entire deleted region using YACs, PACs, and BACs. Five overlapping cosmid clones derived from YAC clones completely covered the entire deleted region: its size was estimated to be no larger than 200 kb. We further performed two-color fluorescence in situ hybridization (FISH) analysis to confirm the deletion and narrowed down the deleted region to 100 kb or less; it was covered by three overlapping cosmid clones that were included in one BAC clone. Restriction endonuclease mapping identified a region in which Notl, Sail, Sinal, and Xhol were clustered, suggesting the possible existence of a CpG island.

Original languageEnglish
Pages (from-to)74-77
Number of pages4
JournalGenes Chromosomes and Cancer
Volume23
Issue number1
DOIs
Publication statusPublished - 1998 Sep 1

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Fingerprint Dive into the research topics of 'Identification of a 100-kb region of common allelic loss on chromosome bands 10q25-q26 in human endometrial cancer'. Together they form a unique fingerprint.

  • Cite this

    Yamakawa, H., Nagase, S., Yuki, M., Shiwaku, H. O., Furukawa, T., Yoshinaga, K., Soeda, E., Hoshi, M., Hayashi, Y., Sato, S., Yajima, A., & Horii, A. (1998). Identification of a 100-kb region of common allelic loss on chromosome bands 10q25-q26 in human endometrial cancer. Genes Chromosomes and Cancer, 23(1), 74-77. https://doi.org/10.1002/(SICI)1098-2264(199809)23:1<74::AID-GCC12>3.0.CO;2-E