Identification, cDNA cloning, and mRNA localization of a zebrafish ortholog of leukemia inhibitory factor

Takashi Abe, Tsuyoshi Mikekado, Satoru Haga, Yuya Kisara, Kohei Watanabe, Tadahide Kurokawa, Tohru Suzuki

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Leukemia inhibitory factor (LIF) maintains embryonic stem cells in an undifferentiated state. To establish stable cultures of zebrafish embryonic stem cells, recombinant zebrafish LIF (zLIF) is needed because the LIF sequence varies greatly between species. In the current study, we identified the zebrafish (Danio rerio) and pufferfish (Tetraodon nigroviridis) orthologs of lif from genomic databases, and we isolated a cDNA encoding zLIF. Synteny analysis and comparison of sequences identified zebrafish and Tetraodon orthologs of human LIF. The cDNA for zLIF encoded a predicted 215-amino acid protein with a putative 32-amino acid signal peptide, two disulfide bonds, and two N-linked glycosylation sites. We found that transcription of zlif starts at the hatching period during embryogenesis and is present in the brain, visceral organs, bone, and skin.

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Issue number1
Publication statusPublished - 2007 May


  • Cytokine
  • Leukemia inhibitory factor
  • Synteny
  • Tetraodon
  • Zebrafish
  • cDNA

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology


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