TY - JOUR
T1 - Identification, cDNA cloning, and mRNA localization of a zebrafish ortholog of leukemia inhibitory factor
AU - Abe, Takashi
AU - Mikekado, Tsuyoshi
AU - Haga, Satoru
AU - Kisara, Yuya
AU - Watanabe, Kohei
AU - Kurokawa, Tadahide
AU - Suzuki, Tohru
N1 - Funding Information:
This study was partially supported by a grant-in-aid (Bio-Design Program) to T.K. from the Ministry of Agriculture, Forestry, and Fisheries.
PY - 2007/5
Y1 - 2007/5
N2 - Leukemia inhibitory factor (LIF) maintains embryonic stem cells in an undifferentiated state. To establish stable cultures of zebrafish embryonic stem cells, recombinant zebrafish LIF (zLIF) is needed because the LIF sequence varies greatly between species. In the current study, we identified the zebrafish (Danio rerio) and pufferfish (Tetraodon nigroviridis) orthologs of lif from genomic databases, and we isolated a cDNA encoding zLIF. Synteny analysis and comparison of sequences identified zebrafish and Tetraodon orthologs of human LIF. The cDNA for zLIF encoded a predicted 215-amino acid protein with a putative 32-amino acid signal peptide, two disulfide bonds, and two N-linked glycosylation sites. We found that transcription of zlif starts at the hatching period during embryogenesis and is present in the brain, visceral organs, bone, and skin.
AB - Leukemia inhibitory factor (LIF) maintains embryonic stem cells in an undifferentiated state. To establish stable cultures of zebrafish embryonic stem cells, recombinant zebrafish LIF (zLIF) is needed because the LIF sequence varies greatly between species. In the current study, we identified the zebrafish (Danio rerio) and pufferfish (Tetraodon nigroviridis) orthologs of lif from genomic databases, and we isolated a cDNA encoding zLIF. Synteny analysis and comparison of sequences identified zebrafish and Tetraodon orthologs of human LIF. The cDNA for zLIF encoded a predicted 215-amino acid protein with a putative 32-amino acid signal peptide, two disulfide bonds, and two N-linked glycosylation sites. We found that transcription of zlif starts at the hatching period during embryogenesis and is present in the brain, visceral organs, bone, and skin.
KW - Cytokine
KW - Leukemia inhibitory factor
KW - Synteny
KW - Tetraodon
KW - Zebrafish
KW - cDNA
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U2 - 10.1016/j.cbpb.2006.12.019
DO - 10.1016/j.cbpb.2006.12.019
M3 - Article
C2 - 17307004
AN - SCOPUS:33947574742
VL - 147
SP - 38
EP - 44
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 1096-4959
IS - 1
ER -