Identification and distinct regulation of three di/tripeptide transporters in Aspergillus oryzae

Mizuki Tanaka, Keisuke Ito, Tomomi Matsuura, Yasuaki Kawarasaki, Katsuya Gomi

Research output: Contribution to journalArticlepeer-review

Abstract

The uptake of di/tripeptides is mediated by the proton-dependent oligopeptide transporter (POT) family. In this study, 3 POT family transporters, designated PotA, PotB, and PotC were identified in Aspergillus oryzae. Growth comparison of deletion mutants of these transporter genes suggested that PotB and PotC are responsible for di/tripeptide uptake. PotA, which had the highest sequence similarity to yeast POT (Ptr2), contributed little to the uptake. Nitrogen starvation induced potB and potC expression, but not potA expression. When 3 dipeptides were provided as nitrogen sources, the expression profiles of these genes were different. PrtR, a transcription factor that regulates proteolytic genes, was involved in regulation of potA and potB but not in potC expression. Only potC expression levels were dramatically reduced by disruption of ubrA, an orthologue of yeast ubiquitin ligase UBR1 responsible for PTR2 expression. Expression of individual POT genes is apparently controlled by different regulatory mechanisms.

Original languageEnglish
Pages (from-to)452-463
Number of pages12
JournalBioscience, Biotechnology, and Biochemistry
Volume85
Issue number2
DOIs
Publication statusPublished - 2021 Feb 18

Keywords

  • Aspergillus oryzae
  • gene regulation
  • peptide transporter
  • proteolytic gene
  • transcription factor

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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