Identification and detection of prokaryotic symbionts in the ciliate metopus from anaerobic granular sludge

Yuga Hirakata, Mamoru Oshiki, Kyohei Kuroda, Masashi Hatamoto, Kengo Kubota, Takashi Yamaguchi, Hideki Harada, Nobuo Araki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The aim of the present study was to investigate the prokaryotic community structure of the anaerobic ciliate, Metopus sp. using rRNA sequencing, fluorescence in situ hybridization (FISH), and transmission electron microscopy (TEM). Metopus sp. was physically separated from anaerobic granular sludge in a domestic wastewater treatment plant and anoxically cultivated for 7 d. 16S rRNA gene sequences from the prokaryotes Methanoregula boonei and Clostridium aminobutyricum were abundantly detected in Metopus ciliates. The FISH analysis using the oligonucleotide probes Mg1200b and Cla568 demonstrated that these prokaryotes were localized within Metopus cells. These results identify M. boonei- and C. aminobutyricum-like prokaryotes as novel endosymbionts of Metopus ciliates.

Original languageEnglish
Pages (from-to)335-338
Number of pages4
JournalMicrobes and environments
Volume30
Issue number4
DOIs
Publication statusPublished - 2015 Dec 22

Keywords

  • Anaerobic granular sludge
  • Clostridium aminobutyricum
  • Endosymbionts
  • Methanoregula boonei
  • Metopus ciliates

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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