Abstract
A Pseudomonas TOL plasmid pWW0 possesses toluene degradative pathway (xyl) genes. Unstable maintenance of a pWW0 derivative in Escherichia coli allowed us to identify two transposable elements each carrying all the xyl genes. One element corresponded to a 56 kb transposon, Tn 4651, which we had previously characterized. The other element newly identified in this study was 70 kb long, and this element, designated Tn 4653, completely included Tn 4651. Genetic analysis of Tn 4653 demonstrated that its transposition involves two steps, i.e. cointegrate formation and its subsequent resolution. The former step required a trans-acting factor, transposase, which was encoded in a 3.0 kb fragment at one end of Tn 4653, and the latter step was inferred to be mediated by the factors necessary for resolution of the Tn 4651-mediated cointegrate. The transposase functions were not interchangeable between the two transposons.
| Original language | English |
|---|---|
| Pages (from-to) | 72-77 |
| Number of pages | 6 |
| Journal | MGG Molecular & General Genetics |
| Volume | 213 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1988 Jul 1 |
| Externally published | Yes |
Keywords
- TOL plasmid
- Tn 4651
- Tn 4653
- Toluene degrading genes
- Transposon
ASJC Scopus subject areas
- Genetics