TY - JOUR
T1 - Identification and characterization of Tn 4653, a transposon covering the toluene transposon Tn 4651 on TOL plasmid pWW0
AU - Tsuda, Masataka
AU - Iino, Tetsuo
PY - 1988/7/1
Y1 - 1988/7/1
N2 - A Pseudomonas TOL plasmid pWW0 possesses toluene degradative pathway (xyl) genes. Unstable maintenance of a pWW0 derivative in Escherichia coli allowed us to identify two transposable elements each carrying all the xyl genes. One element corresponded to a 56 kb transposon, Tn 4651, which we had previously characterized. The other element newly identified in this study was 70 kb long, and this element, designated Tn 4653, completely included Tn 4651. Genetic analysis of Tn 4653 demonstrated that its transposition involves two steps, i.e. cointegrate formation and its subsequent resolution. The former step required a trans-acting factor, transposase, which was encoded in a 3.0 kb fragment at one end of Tn 4653, and the latter step was inferred to be mediated by the factors necessary for resolution of the Tn 4651-mediated cointegrate. The transposase functions were not interchangeable between the two transposons.
AB - A Pseudomonas TOL plasmid pWW0 possesses toluene degradative pathway (xyl) genes. Unstable maintenance of a pWW0 derivative in Escherichia coli allowed us to identify two transposable elements each carrying all the xyl genes. One element corresponded to a 56 kb transposon, Tn 4651, which we had previously characterized. The other element newly identified in this study was 70 kb long, and this element, designated Tn 4653, completely included Tn 4651. Genetic analysis of Tn 4653 demonstrated that its transposition involves two steps, i.e. cointegrate formation and its subsequent resolution. The former step required a trans-acting factor, transposase, which was encoded in a 3.0 kb fragment at one end of Tn 4653, and the latter step was inferred to be mediated by the factors necessary for resolution of the Tn 4651-mediated cointegrate. The transposase functions were not interchangeable between the two transposons.
KW - TOL plasmid
KW - Tn 4651
KW - Tn 4653
KW - Toluene degrading genes
KW - Transposon
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U2 - 10.1007/BF00333400
DO - 10.1007/BF00333400
M3 - Article
C2 - 2851712
AN - SCOPUS:0024046404
VL - 213
SP - 72
EP - 77
JO - Zeitschrift für Induktive Abstammungs- und Vererbungslehre
JF - Zeitschrift für Induktive Abstammungs- und Vererbungslehre
SN - 1617-4615
IS - 1
ER -