Identification and characterization of Tn 4653, a transposon covering the toluene transposon Tn 4651 on TOL plasmid pWW0

Masataka Tsuda, Tetsuo Iino

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56 Citations (Scopus)

Abstract

A Pseudomonas TOL plasmid pWW0 possesses toluene degradative pathway (xyl) genes. Unstable maintenance of a pWW0 derivative in Escherichia coli allowed us to identify two transposable elements each carrying all the xyl genes. One element corresponded to a 56 kb transposon, Tn 4651, which we had previously characterized. The other element newly identified in this study was 70 kb long, and this element, designated Tn 4653, completely included Tn 4651. Genetic analysis of Tn 4653 demonstrated that its transposition involves two steps, i.e. cointegrate formation and its subsequent resolution. The former step required a trans-acting factor, transposase, which was encoded in a 3.0 kb fragment at one end of Tn 4653, and the latter step was inferred to be mediated by the factors necessary for resolution of the Tn 4651-mediated cointegrate. The transposase functions were not interchangeable between the two transposons.

Original languageEnglish
Pages (from-to)72-77
Number of pages6
JournalMGG Molecular & General Genetics
Volume213
Issue number1
DOIs
Publication statusPublished - 1988 Jul 1
Externally publishedYes

Keywords

  • TOL plasmid
  • Tn 4651
  • Tn 4653
  • Toluene degrading genes
  • Transposon

ASJC Scopus subject areas

  • Genetics

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