Identification and characterization of the two isoforms of the vertebrate H2A.Z histone variant

Ryo Matsuda, Tetsuya Hori, Hiroshi Kitamura, Kozo Takeuchi, Tatsuo Fukagawa, Masahiko Harata

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

Histone variants play important roles in the epigenetic regulation of genome function. The histone variant H2A.Z is evolutionarily conserved from yeast to vertebrates, and it has been reported to have multiple effects upon gene expression and insulation, and chromosome segregation. Recently two genes encoding H2A.Z were identified in the vertebrate genome. However, it is not yet clear whether the proteins transcribed from these genes are functionally distinct. To address this issue, we knocked out each gene individually in chicken DT40 cells. We found that two distinct proteins, H2A.Z-1 and H2A.Z-2, were produced from these genes, and that these proteins could be separated on a long SDS-PAGE gel. The two isoforms were deposited to a similar extent by the SRCAP chromatin-remodeling complex, suggesting redundancy to their function. However, cells lacking either one of the two isoforms exhibited distinct alterations in cell growth and gene expression, suggesting that the two isoforms have differential effects upon nucleo-some stability and chromatin structure. These findings provide insight into the molecular basis of the multiple functions of the H2A.Z gene products.

Original languageEnglish
Article numbergkq171
Pages (from-to)4263-4273
Number of pages11
JournalNucleic acids research
Volume38
Issue number13
DOIs
Publication statusPublished - 2010 Mar 18

ASJC Scopus subject areas

  • Genetics

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