Identification and characterization of multiple β-glucan binding proteins in the Pacific oyster, Crassostrea gigas

Naoki Itoh, Ryo Kamitaka, Keisuke G. Takahashi, Makoto Osada

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The present study reports on the characterization of two cDNAs coding β-glucan binding proteins (βGBPs), designated as Cg-βGBP-1 and Cg-βGBP-2, from the Pacific oyster, Crassostrea gigas. Cg-βGBP-1 consists of 555 amino acid residues and possesses two possible integrin recognition sites. The other protein, Cg-βGBP-2, is composed of 447 amino acid residues without integrin recognition sites. Domain structures of both Cg-βGBPs are similar to other invertebrate βGBPs, but phylogenetic positions and major expression tissues for these proteins are different. Cg-βGBP-1 is expressed in circulatory hemocytes and Cg-βGBP-2 in digestive glands. Functional assays using recombinant proteins revealed that Cg-βGBP-2 enhanced the phenoloxidase (PO) activity of hemocyte suspensions under the presence of laminarin, but Cg-βGBP-1 did not show this enhancement. It is suggested that Cg-βGBPs in the Pacific oyster have evolved to obtain different immunological functions. Cg-βGBP-1 possibly evolved for hemocyte-related functions through integrin, and Cg-βGBP-2 for the PO activation system.

Original languageEnglish
Pages (from-to)445-454
Number of pages10
JournalDevelopmental and Comparative Immunology
Volume34
Issue number4
DOIs
Publication statusPublished - 2010 Apr 1

Keywords

  • Gene expression
  • Hemocytes
  • Innate immunity
  • Pacific oyster
  • Pattern recognition receptors (PRRs)
  • Phenoloxidase
  • Recombinant protein
  • β-Glucan binding proteins

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

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