TY - JOUR
T1 - Identification and Characterization of IgE-Reactive Proteins and a New Allergen (Cic a 1.01) from Chickpea (Cicer arietinum)
AU - Wangorsch, Andrea
AU - Kulkarni, Anuja
AU - Jamin, Annette
AU - Spiric, Jelena
AU - Bräcker, Julia
AU - Brockmeyer, Jens
AU - Mahler, Vera
AU - Blanca-López, Natalia
AU - Ferrer, Marta
AU - Blanca, Miguel
AU - Torres, Maria
AU - Gomez, Paqui
AU - Bartra, Joan
AU - García-Moral, Alba
AU - Goikoetxea, María J.
AU - Vieths, Stefan
AU - Toda, Masako
AU - Zoccatelli, Gianni
AU - Scheurer, Stephan
N1 - Funding Information:
The authors gratefully thank Anna-Maria Engin for excellent technical assistance in MS analysis. This work was supported by Laxmi Ananthanarayan and by the UGC-SAP (BSR) fellowship for Doctoral candidates, India; DAAD fellowship for Doctoral candidates, Germany; Instituto de Salud Carlos III (ISCIII) co-founded by Fondo Europeo de Desarrollo Regional?FEDER for the Thematic Networks and Co-operative Research Centres: ARADyAL (RD16/0006/0024, RD16/0006/0007, RD16/0006/0031)?.
Funding Information:
The authors gratefully thank Anna‐Maria Engin for excellent technical assistance in MS analysis. This work was supported by Laxmi Ananthanarayan and by the UGC‐SAP (BSR) fellowship for Doctoral candidates, India; DAAD fellowship for Doctoral candidates, Germany; Instituto de Salud Carlos III (ISCIII) co‐founded by Fondo Europeo de Desarrollo Regional—FEDER for the Thematic Networks and Co‐operative Research Centres: ARADyAL (RD16/0006/0024, RD16/0006/0007, RD16/0006/0031)”.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Scope: Chickpea (Cicer arietinum) allergy has frequently been reported particularly in Spain and India. Nevertheless, chickpea allergens are poorly characterized. The authors aim to identify and characterize potential allergens from chickpea. Methods and Results: Candidate proteins are selected by an in silico approach or immunoglobuline E (IgE)-testing. Potential allergens are prepared as recombinant or natural proteins and characterized for structural integrity by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), circular dichroism (CD)-spectroscopy, and mass spectrometry (MS) analysis. IgE-sensitization pattern of Spanish chickpea allergic and German peanut and birch pollen sensitized patients are investigated using chickpea extracts and purified proteins. Chickpea allergic patients show individual and heterogeneous IgE-sensitization profiles with extracts from raw and boiled chickpeas. Chickpea proteins pathogenesis related protein family 10 (PR-10), a late embryogenesis abundant protein (LEA/DC-8), and a vicilin-containing fraction, but not 2S albumin, shows IgE reactivity with sera from chickpea, birch pollen, and peanut sensitized patients. Remarkably, allergenic vicilin, DC-8, and PR-10 are detected in the extract of boiled chickpeas. Conclusion: Several IgE-reactive chickpea allergens are identified. For the first time a yet not classified DC-8 protein is characterized as minor allergen (Cic a 1). Finally, the data suggest a potential risk for peanut allergic patients by IgE cross-reactivity with homologous chickpea proteins.
AB - Scope: Chickpea (Cicer arietinum) allergy has frequently been reported particularly in Spain and India. Nevertheless, chickpea allergens are poorly characterized. The authors aim to identify and characterize potential allergens from chickpea. Methods and Results: Candidate proteins are selected by an in silico approach or immunoglobuline E (IgE)-testing. Potential allergens are prepared as recombinant or natural proteins and characterized for structural integrity by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), circular dichroism (CD)-spectroscopy, and mass spectrometry (MS) analysis. IgE-sensitization pattern of Spanish chickpea allergic and German peanut and birch pollen sensitized patients are investigated using chickpea extracts and purified proteins. Chickpea allergic patients show individual and heterogeneous IgE-sensitization profiles with extracts from raw and boiled chickpeas. Chickpea proteins pathogenesis related protein family 10 (PR-10), a late embryogenesis abundant protein (LEA/DC-8), and a vicilin-containing fraction, but not 2S albumin, shows IgE reactivity with sera from chickpea, birch pollen, and peanut sensitized patients. Remarkably, allergenic vicilin, DC-8, and PR-10 are detected in the extract of boiled chickpeas. Conclusion: Several IgE-reactive chickpea allergens are identified. For the first time a yet not classified DC-8 protein is characterized as minor allergen (Cic a 1). Finally, the data suggest a potential risk for peanut allergic patients by IgE cross-reactivity with homologous chickpea proteins.
KW - Cic a 1
KW - Cicer arietinum
KW - IgE cross-reactivity
KW - allergen
KW - chickpea allergy
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U2 - 10.1002/mnfr.202000560
DO - 10.1002/mnfr.202000560
M3 - Article
C2 - 32875712
AN - SCOPUS:85091151730
VL - 64
JO - Die Nahrung
JF - Die Nahrung
SN - 1613-4125
IS - 19
M1 - 2000560
ER -