Identification and Characterization of IgE-Reactive Proteins and a New Allergen (Cic a 1.01) from Chickpea (Cicer arietinum)

Andrea Wangorsch, Anuja Kulkarni, Annette Jamin, Jelena Spiric, Julia Bräcker, Jens Brockmeyer, Vera Mahler, Natalia Blanca-López, Marta Ferrer, Miguel Blanca, Maria Torres, Paqui Gomez, Joan Bartra, Alba García-Moral, María J. Goikoetxea, Stefan Vieths, Masako Toda, Gianni Zoccatelli, Stephan Scheurer

Research output: Contribution to journalArticlepeer-review

Abstract

Scope: Chickpea (Cicer arietinum) allergy has frequently been reported particularly in Spain and India. Nevertheless, chickpea allergens are poorly characterized. The authors aim to identify and characterize potential allergens from chickpea. Methods and Results: Candidate proteins are selected by an in silico approach or immunoglobuline E (IgE)-testing. Potential allergens are prepared as recombinant or natural proteins and characterized for structural integrity by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), circular dichroism (CD)-spectroscopy, and mass spectrometry (MS) analysis. IgE-sensitization pattern of Spanish chickpea allergic and German peanut and birch pollen sensitized patients are investigated using chickpea extracts and purified proteins. Chickpea allergic patients show individual and heterogeneous IgE-sensitization profiles with extracts from raw and boiled chickpeas. Chickpea proteins pathogenesis related protein family 10 (PR-10), a late embryogenesis abundant protein (LEA/DC-8), and a vicilin-containing fraction, but not 2S albumin, shows IgE reactivity with sera from chickpea, birch pollen, and peanut sensitized patients. Remarkably, allergenic vicilin, DC-8, and PR-10 are detected in the extract of boiled chickpeas. Conclusion: Several IgE-reactive chickpea allergens are identified. For the first time a yet not classified DC-8 protein is characterized as minor allergen (Cic a 1). Finally, the data suggest a potential risk for peanut allergic patients by IgE cross-reactivity with homologous chickpea proteins.

Original languageEnglish
Article number2000560
JournalMolecular Nutrition and Food Research
Volume64
Issue number19
DOIs
Publication statusPublished - 2020 Oct 1

Keywords

  • Cic a 1
  • Cicer arietinum
  • IgE cross-reactivity
  • allergen
  • chickpea allergy

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

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