Identification and characterization of apoptosis regulator Bax involved in air-exposure stress of the mud crab, Scylla Paramamosain Estampador, 1949

Wen Bin Gu, Yi Lian Zhou, Dan Dan Tu, Zhong Kai Zhou, Qi Hui Zhu, Guang Xu Liu, Miao An Shu

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The mud crab, Scylla paramamosain Estampador, 1949, is a widely farmed commercial species in the South-East coastal areas of China. The crabs are captured and placed in a water-free container for further transportation. The long-time air exposure makes the crabs suffer from oxidative stress, triggering cell apoptosis and leading to the crab's death. In this study, the homologue of the apoptosis regulator BAX was firstly identified in S. paramamosain and named as SpBAX. The coding region of SpBAX yielded a polypeptide of 278 amino acids, consisting of the defining motif of the BAXs family including Bcl-2 homologous BH1, BH2, BH3 regions and a transmembrane (TM) domain. Subcellular prediction suggested that SpBAX was located in the cytoplasm. The three-dimensional structure showed that SpBAX contained 8 helical regions. Two central -helices (5 and 6) flanked on one side by 3 and 4, and on the other side by 1, 2 and 7, which showed the same structure as BAXs in mammals. The highest expression level of SpBAX was detected in hepatopancreas tissue. The expression level of SpBAX was up-regulated in 12 h in hepatopancreas and in 24 h in haemocytes after air exposure. Meanwhile, a flow cytometry assay revealed that the proportion of apoptotic haemocytes exceeded 65% after air exposure for 36 h, showing a significant difference with the control group. These results indicated that the cloned SpBAX might be involved in the response to air exposure stress by causing cell apoptosis. This study may thus be helpful to clarify the mechanisms of the air-exposure stress response in the mud crab.

Original languageEnglish
Pages (from-to)1373-1390
Number of pages18
JournalCrustaceana
Volume90
Issue number11-12
DOIs
Publication statusPublished - 2017
Externally publishedYes

ASJC Scopus subject areas

  • Aquatic Science
  • Animal Science and Zoology

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