Identification and characterization of a novel secreted antigen 1 of Babesia microti and evaluation of its potential use in enzyme-linked immunosorbent assay and immunochromatographic test

Yuzi Luo, Honglin Jia, M. Alaa Terkawi, Youn Kyoung Goo, Suguru Kawano, Hideo Ooka, Yan Li, Longzheng Yu, Shinuo Cao, Junya Yamagishi, Kozo Fujisaki, Yoshifumi Nishikawa, Atsuko Saito-Ito, Ikuo Igarashi, Xuenan Xuan

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    30 Citations (Scopus)

    Abstract

    Here, we identified a novel secreted antigen designated as Babesia microti secreted antigen 1 (BmSA1) by immunoscreening a B. microti cDNA expression library using the sera from hamsters immunized with plasma, putatively containing secreted antigens, from B. microti-infected hamsters. Antibodies raised in mice immunized with recombinant BmSA1 (r. BmSA1) recognized a native 33-kDa parasite protein. An enzyme-linked immunosorbent assay (ELISA) of r. BmSA1 detected specific antibodies as early as 6 and 4. days post-infection in sera from a hamster experimentally infected with B. microti Gray strain (US type) and a mouse experimentally infected with B. microti Munich strain (rodent isolate), respectively. Moreover, a rapid immunochromatographic test (ICT) using r. BmSA1 detected specific antibodies in a hamster experimentally infected with B. microti from day 6 to at least day 270 post-infection, which was quite consistent with the results of the ELISA. In addition, analysis of the sera involved in the first case of human babesiosis in Japan (Kobe type) showed that specific antibodies were detectable in the patient and the positive donor by ELISA using r. BmSA1, and the ICT result was identical to the ELISA data. Taken together, these results indicated that BmSA1 could be a promising and universal target for developing both ELISA and ICT for the serodiagnosis of human babesiosis and for an epidemiological survey of its rodent reservoir.

    Original languageEnglish
    Pages (from-to)119-125
    Number of pages7
    JournalParasitology International
    Volume60
    Issue number2
    DOIs
    Publication statusPublished - 2011 Jun

    Keywords

    • Babesia microti
    • ELISA
    • Immunochromatographic test
    • Secreted antigen 1

    ASJC Scopus subject areas

    • Parasitology
    • Infectious Diseases

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  • Cite this

    Luo, Y., Jia, H., Terkawi, M. A., Goo, Y. K., Kawano, S., Ooka, H., Li, Y., Yu, L., Cao, S., Yamagishi, J., Fujisaki, K., Nishikawa, Y., Saito-Ito, A., Igarashi, I., & Xuan, X. (2011). Identification and characterization of a novel secreted antigen 1 of Babesia microti and evaluation of its potential use in enzyme-linked immunosorbent assay and immunochromatographic test. Parasitology International, 60(2), 119-125. https://doi.org/10.1016/j.parint.2010.11.001