Identification and characterization of a novel bacterial β-glucosidase that is highly specific for the β-1,2-glucosidic linkage of sesaminol triglucoside

Akinori Sakurai, Shuntaro Hongo, Arun Nair, Toshiyuki Waki, Daiki Oikawa, Takuma Nishio, Takefumi Shimoyama, Seiji Takahashi, Satoshi Yamashita, Toru Nakayama

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A gene (PSTG2) coding for a novel β-glucosidase belonging to glycoside hydrolase family 3 was identified in the vicinity of the previously identified β-glucosidase gene [sesaminol triglucoside (STG)-hydrolyzing β-glucosidase, PSTG1] in the genome of Paenibacillus sp. strain KB0549. Compared with PSTG1, recombinant PSTG2 more specifically acted on the β-1,2-glucosidic linkage of the STG molecule to transiently accumulate a larger amount of 6-O-(β-D-glucopyranosyl)-β-D-glucopyranosylsesaminol.

Original languageEnglish
Pages (from-to)1518-1521
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume82
Issue number9
DOIs
Publication statusPublished - 2018

Keywords

  • Glycoside hydrolase family 3
  • Paenibacillus
  • Sesaminol triglucoside
  • Β-glucosidase

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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