Identification and characterization of 2'-deoxyuridine from the supernatant of conidial suspensions of rice blast fungus as an infection-promoting factor in rice plants

Sugihiro Ando, Yuko Sato, Hideyuki Shigemori, Takafumi Shimizu, Kazunori Okada, Hisakazu Yamane, Yusuke Jikumaru, Yuji Kamiya, Kosumi Yamada, Chiharu Akimoto-Tomiyama, Shigeru Tanabe, Yoko Nishizawa, Eiichi Minami

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We previously detected infection-promoting activity in the supernatant of the conidial suspension (SCS) of the rice blast fungus. In the present study, a molecule carrying the activity was purified and identified as 2'-deoxyuridine(dU). The infection-promoting activity of dU was strictly dependent on its chemical structure and displayed characteristics consistent with those of the SCS. Notably, the activity of dU was exclusively detected during interactions between rice and virulent isolates of the fungus, the number of susceptible lesions in leaf blades was increased by dU, and nonhost resistance in rice plants was not affected by treatment with dU. In addition, the expression of pathogensis-related genes, accumulation of H 2O 2, and production of phytoalexins in rice in response to inoculation with virulent fungal isolates was not suppressed by dU. The infection-promoting activity of dU was not accompanied by elevated levels of endogenous abscissic acid, which is known to modify plant-pathogen interactions, and was not detected in interactions between oat plants and a virulent oat blast fungus isolate. Taken together, these results demonstrate that dU is a novel infection-promoting factor that acts specifically during compatible interactions between rice plants and rice blast fungus in a mode distinct from that of toxins and suppressors.

Original languageEnglish
Pages (from-to)519-532
Number of pages14
JournalMolecular Plant-Microbe Interactions
Volume24
Issue number5
DOIs
Publication statusPublished - 2011 May

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

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