Hypoxia triggers collective aerotactic migration in dictyostelium discoideum

Olivier Cochet-Escartin, Mete Demircigil, Satomi Hirose, Blandine Allais, Philippe Gonzalo, Ivan Mikaelian, Kenichi Funamoto, Christophe Anjard, Vincent Calvez, Jean Paul Rieu

Research output: Contribution to journalArticlepeer-review

Abstract

Using a self-generated hypoxic assay, we show that the amoeba Dictyostelium discoideum displays a remarkable collective aerotactic behavior. When a cell colony is covered, cells quickly consume the available oxygen (O2) and form a dense ring moving outwards at constant speed and density. To decipher this collective process, we combined two technological developments: porphyrin-based O2-sensing films and microfluidic O2 gradient generators. We showed that Dictyostelium cells exhibit aerotactic and aerokinetic response in a low range of O2 concentration indicative of a very efficient detection mechanism. Cell behaviors under self-generated or imposed O2 gradients were modeled using an in silico cellular Potts model built on experimental observations. This computational model was complemented with a parsimonious ‘Go or Grow’ partial differential equation (PDE) model. In both models, we found that the collective migration of a dense ring can be explained by the interplay between cell division and the modulation of aerotaxis.

Original languageEnglish
Article numbere64731
JournaleLife
Volume10
DOIs
Publication statusPublished - 2021 Aug

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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