Hypoxia-inducible factor 1 alpha (HIF-1α) gene expression in human ovarian carcinoma

Kentaro Nakayama, Atsuko Kanzaki, Kohkichi Hata, Hidetaka Katabuchi, Hitoshi Okamura, Kohji Miyazaki, Manabu Fukumoto, Yuji Takebayashi

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62 Citations (Scopus)

Abstract

Hypoxia-inducible factor 1α (HIF-1α) that regulates genes involved in response to hypoxia and promotes neo-angiogenesis, is a transcriptional factor for vascular endothelial cell growth factor (VEGF). The aim of this study was to examine the expression of HIF-1α and VEGF gene expressions and their relation to angiogenesis, clinicopathologic variables and survival in the patient with human ovarian carcinoma. We retrospectively analyzed HIF-1α and VEGF gene expression levels using reverse transcriptase polymerase chain reaction (RT-PCR) in 60 ovarian carcinomas. Intratumoral microvessel density (IMD) was assessed by immunostaining endothelial cells, using anti-CD 31 antibody in frozen sections. The relationships between the expression level of these genes, IMD and clinicopathologic variables were evaluated by Student's t-test and chi-square tests. Survival analysis was performed by Kaplan-Meier curves. HIF-1α or VEGF gene expression level was independent of age, clinical stage and histological subtype besides grade of tumor. There was no relationship between HIF-1α or VEGF gene expression level and IMD in all carcinomas (R=0.118 and 0.224, respectively). In addition, a weak association between HIF-1α and VEGF gene expression level was observed (R=0.300, P=0.020). The association between VEGF gene expression and IMD was observed (R=0.501, P=0.016). However, no association between IMD and HIF-1α gene expression was observed. Further, both HIF-1α and VEGF gene expression levels had no effect on survival in the patient with ovarian carcinoma. These results suggest that VEGF upregulated by HIF-1α gene may be involved in angiogenesis of some type of ovarian carcinoma, but the expression levels of both genes have no effect on survival in the patients with ovarian carcinoma.

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalCancer Letters
Volume176
Issue number2
DOIs
Publication statusPublished - 2002 Feb 25

Keywords

  • Angiogenesis
  • Hypoxia-inducible factor 1 alpha
  • Intratumoral microvessel density
  • Ovarian carcinoma
  • Vascular endothelial cell growth factor

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Nakayama, K., Kanzaki, A., Hata, K., Katabuchi, H., Okamura, H., Miyazaki, K., Fukumoto, M., & Takebayashi, Y. (2002). Hypoxia-inducible factor 1 alpha (HIF-1α) gene expression in human ovarian carcinoma. Cancer Letters, 176(2), 215-223. https://doi.org/10.1016/S0304-3835(01)00762-5