Hypoxia enhances osteogenic differentiation in retinoic acid-treated murine-induced pluripotent stem cells

Jeeranan Manokawinchoke, Thanaphum Osathanon, Hiroshi Egusa, Prasit Pavasant

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Hypoxic condition influences biological responses in various cell types. However, a hypoxic regulating osteogenic differentiation remains controversy. Here, an influence of short-term culture in hypoxic condition on osteogenic marker gene expression by retinoic acid-treated murine gingival fibroblast-derived induced pluripotent stem cells (RA-miPS) was investigated. Results demonstrated that hypoxic condition significantly upregulated Vegf, Runx2, Osx, and Ocn mRNA expression by RA-miPS in normal culture medium at day 3. Further, desferrioxamine significantly downregulated pluripotent marker (Nanog and Oct4) and enhanced osteogenic marker (Runx2, Osx, Dlx5, and Ocn) gene expression as well as promoted in vitro mineral deposition. However, the effect of cobalt chloride on osteogenic differentiation of RA-miPS was not robust. In summary, the results imply that hypoxic condition may be useful in the enhancement of osteogenic differentiation in RA-miPS.

Original languageEnglish
Pages (from-to)547-553
Number of pages7
JournalTissue Engineering and Regenerative Medicine
Volume13
Issue number5
DOIs
Publication statusPublished - 2016 Oct 1

Keywords

  • Cobalt chloride
  • Desferrioxamine
  • Hypoxia
  • Pluripotent stem cells

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

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