Hypoxemia induces expression of heme oxygenase-1 and heme oxygenase-2 proteins in the mouse myocardium

Feng Han, Kazuhisa Takeda, Masao Ono, Fumiko Date, Kazunobu Ishikawa, Satoru Yokoyama, Yotaro Shinozawa, Kazumichi Furuyama, Shigeki Shibahara

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Heme oxygenase (HO) catalyzes oxidative breakdown of heme, and constitutes two isozymes, HO-1 and HO-2. Here, we explored the tissue-specific regulation of expression of HO-1 and HO-2 under hypoxemia. There was no significant change in the overall expression levels of HO-1 and HO-2 mRNAs and proteins in the lung during adaptation of C57BL/6 mice to normobaric hypoxia (10% O2). However, immunohistochemical analysis revealed the increased expression of HO-1 and HO-2 proteins after 28 days of normobaric hypoxia in the pulmonary venous myocardium that is the extension of the left atrial myocardium into pulmonary venous walls. Moreover, the expression of HO-2 protein was increased in the sub-endocardial myocardium of ventricles under hypoxia, while HO-1 protein level was increased in the full-thickness walls. Thus, hypoxemia induces expression of both HO-1 and HO-2 proteins in the myocardium. Using C57BL/6 mice lacking HO-2 (HO-2-/-), which manifest chronic hypoxemia, we also showed that the HO-1 protein level in the lung was similar between HO-2-/- mice and wild-type mice. Unexpectedly, HO-1 protein level was lower by 35% in the HO-2-/- mouse liver than the wild-type liver. These results indicate that the expression of HO-1 protein is regulated in a tissue-specific manner under hypoxemia.

Original languageEnglish
Pages (from-to)143-151
Number of pages9
JournalJournal of biochemistry
Volume147
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1

Keywords

  • Bilirubin
  • Heart
  • Heme oxygenase
  • Hypoxemia
  • Hypoxia
  • Liver
  • Lung
  • Myocardium
  • Oxygen sensor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Han, F., Takeda, K., Ono, M., Date, F., Ishikawa, K., Yokoyama, S., Shinozawa, Y., Furuyama, K., & Shibahara, S. (2010). Hypoxemia induces expression of heme oxygenase-1 and heme oxygenase-2 proteins in the mouse myocardium. Journal of biochemistry, 147(1), 143-151. https://doi.org/10.1093/jb/mvp153