Hypothermia protects cultured human retinal pigment epithelial cells against trypan blue toxicity

Hiroshi Kunikata, Hiroshi Tomita, Hiromi Murata, Yoshiko Sagara, Hajime Sato, Yuko Wada, Nobuo Fuse, Yoichi Nakagawa, Toshiaki Abe, Makoto Tamai

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Purpose: To determine whether trypan blue (TB) is toxic to cultured human retinal pigment epithelial (ARPE-19) cells, and whether hypothermia can protect ARPE-19 cells against TB toxicity. Methods: ARPE-19 cells were cultured and exposed to balanced salt solution as a control, while other cells were exposed to 0.05, 0.2, and 0.5% TB dye at 37 and 4°C for 5 and 30 min. The percentage of ARPE-19 cells that survived was determined by resazurin 1 day after the exposure. Results: A statistically significant decrease in the percentage of ARPE-19 cells surviving was found after exposure to 0.2 and 0.5% TB at any temperature or for any exposure duration (p < 0.01). The percentage of RPE cells surviving at 0.05% was not significantly different from that of controls except for a 30-min exposure at 37°C. The percentage of cells surviving at 4°C for a 5-min exposure to 0.5% TB and a 30-min exposure to 0.2 and 0.5% was significantly higher than that at 37°C under each condition (p < 0.01 for all). Conclusions: These results indicate that TB is toxic to human RPE cells, and the toxicity is dose- and exposure duration-dependent. Exposing the cells at 4°C had a protective effect against higher concentrations or longer exposure durations of TB compared to exposure at 37°C.

Original languageEnglish
Pages (from-to)114-117
Number of pages4
Issue number2
Publication statusPublished - 2006 Feb


  • Hypothermia, protecting effect
  • Retinal pigment epithelial cells
  • Trypan blue toxicity

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems


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