Hypothermia of 8°C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity

Hiroshi Kunikata, Toshiaki Abe, Hiromi Murata, Yoshiko Sagara, Ryosuke Wakusawa, Hajime Sato, Madoka Yoshida, Nobuo Fuse, Makoto Tamai

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

PURPOSE: To determine whether hypothermia of 8°C can protect cultured human retinal pigment epithelial (ARPE-19) cells and rat retinal ganglion cells (RGC-5) against trypan blue (TB) toxicity. DESIGN: Laboratory investigation. METHODS: ARPE-19 cells and RGC-5 were exposed to balanced salt solution as controls, and 0.05% and 0.5% TB at 37°C, and at 8°C for one minute. The percentage of surviving cells was determined by the resazurin test. RESULTS: TB induced a statistically significant decrease in the percentage of ARPE-19 cells surviving at 0.5% TB at 37°C (P < .01). Conversely, TB induced a statistically significant decrease in the percentage of RGC-5 surviving at all conditions except for 0.05% TB at 8°C (0.05% 37°C; P < .05, 0.5% 37°C and 8°C; P < .01). CONCLUSIONS: These results indicate that reducing the temperature to 8°C has a protective effect against the TB toxicity for ARPE-19 cells and RGC-5 in culture.

Original languageEnglish
Pages (from-to)754-756
Number of pages3
JournalAmerican Journal of Ophthalmology
Volume141
Issue number4
DOIs
Publication statusPublished - 2006 Apr

ASJC Scopus subject areas

  • Ophthalmology

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