Hyperosmotic medium partially restores the growth defect and the impaired production of sterigmatocystin of an Aspergillus nidulans ΔpmtC mutant in a HogA-independent manner

Thi Huynh Tram Le, Thy Nhan Le, Akira Yoshimi, Keietsu Abe, Yumi Imanishi-Shimizu, Kiminori Shimizu

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The protein O-mannosyltransferase catalyzes O-mannosylation in the endoplasmic reticulum by transferring mannose to the seryl or threonyl residues of substrate proteins. We previously reported a deletion mutant of O-mannosyltransferase C (ΔpmtC) in Aspergillus nidulans with impaired vegetative growth and sterigmatocystin (ST) production. In this study, we investigated whether osmotic conditions contribute to the developmental processes and ST biosynthesis of the ΔpmtC deletion mutant. We found that hyphal growth and ST production partially improved in the presence of NaCl, KCl or sorbitol as osmotic stabilizers. Conidiation of the ΔpmtC deletion mutant was not restored under osmotic stress conditions when the hogA gene was deleted. The hogA gene encodes a protein required for the cellular response to osmotic pressure. However, the yield of ST and the vegetative growth of the ΔhogA ΔpmtC double deletant was restored by high osmolarity in a HogA-independent manner.

    Original languageEnglish
    Article numberfnab127
    JournalFEMS Microbiology Letters
    Volume368
    Issue number18
    DOIs
    Publication statusPublished - 2021 Sep 1

    Keywords

    • HOG pathway
    • filamentous fungus
    • secondary metabolism

    ASJC Scopus subject areas

    • Microbiology
    • Molecular Biology
    • Genetics

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