TY - JOUR
T1 - Hydrogel electrodeposition based on bipolar electrochemistry
AU - Ino, Kosuke
AU - Matsumoto, Tomoaki
AU - Taira, Noriko
AU - Kumagai, Tatsuki
AU - Nashimoto, Yuji
AU - Shiku, Hitoshi
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (A) (No. 16H02280), a Grant-in-Aid for Scientific Research (B) (No. 15H03542, 18H01840 and 18H01999), a Grant-in-Aid for Challenging Exploratory Research (No. 16K14012), and a Grant-in-Aid for Young Scientists (A) (No. 15H05415) from the Japan Society for the Promotion of Science (JSPS). This work was also supported by the Asahi Glass Foundation. K. I. thanks a program of ALicE in Tohoku University. The authors thank Prof. Tomokazu Matsue (Tohoku University) for his assistance with the experimental equipment.
PY - 2018/8/21
Y1 - 2018/8/21
N2 - Bipolar electrochemistry has attracted great interest for applications based on sensing, electrografting, and electrodeposition, because the technique enables electrochemical reactions to be induced at multiple bipolar electrodes (BPEs) with only a single power supply. However, there are only a few reports on the biofabrication of hydrogels using BPEs. In this study, we applied bipolar electrochemistry to achieve the electrodeposition of calcium-alginate hydrogels at specified target areas, which is possible because of the use of water electrolysis to obtain acidification at the anodic pole. This scheme was used to successfully fabricate an array of hydrogel deposits at a BPE array. In addition, hydrogels were successfully fabricated either at only the target BPEs or only the target areas of BPEs by repositioning the driving electrodes. Furthermore, a hydrogel was drawn on a large BPE as a canvas by using small driving electrodes. As a demonstration of the electrodeposited hydrogels for bioapplications, mammal cells were cultured in the hydrogels. Because the amount and shape of the hydrogel deposits can be controlled by using the bipolar system, the system we developed can be used for biosensors and cell culture platforms.
AB - Bipolar electrochemistry has attracted great interest for applications based on sensing, electrografting, and electrodeposition, because the technique enables electrochemical reactions to be induced at multiple bipolar electrodes (BPEs) with only a single power supply. However, there are only a few reports on the biofabrication of hydrogels using BPEs. In this study, we applied bipolar electrochemistry to achieve the electrodeposition of calcium-alginate hydrogels at specified target areas, which is possible because of the use of water electrolysis to obtain acidification at the anodic pole. This scheme was used to successfully fabricate an array of hydrogel deposits at a BPE array. In addition, hydrogels were successfully fabricated either at only the target BPEs or only the target areas of BPEs by repositioning the driving electrodes. Furthermore, a hydrogel was drawn on a large BPE as a canvas by using small driving electrodes. As a demonstration of the electrodeposited hydrogels for bioapplications, mammal cells were cultured in the hydrogels. Because the amount and shape of the hydrogel deposits can be controlled by using the bipolar system, the system we developed can be used for biosensors and cell culture platforms.
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U2 - 10.1039/c8lc00465j
DO - 10.1039/c8lc00465j
M3 - Article
C2 - 29978172
AN - SCOPUS:85051338868
VL - 18
SP - 2425
EP - 2432
JO - Lab on a Chip - Miniaturisation for Chemistry and Biology
JF - Lab on a Chip - Miniaturisation for Chemistry and Biology
SN - 1473-0197
IS - 16
ER -