Hydrogel culture dish integrated with organic electrodes for efficient stimulation of hiPSC-derived cardiomyocytes

Kensuke Sumomozawa, Kuniaki Nagamine, Hiroyuki Kai, Shotaro Yoshida, Hirokazu Kaji, Matsuhiko Nishizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We propose a hydrogel-based bioreactor integrated with organic electrodes for efficient electrical stimulation of cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs). The bioreactor was fabricated by integration of poly(3,4-ethylenedioxythiophene) (PEDOT)/polyurethane (PU) electrodes and a hydrogel with a cell-positioning chamber. The fabricated organic electrodes had a high capacity compared to conventional metal electrodes that prevented unfavorable electric polarization. Cardiomyocytes derived from hiPSCs were cultured in the bioreactor, and their beating frequency was synchronized to frequency of electrical stimulation applied by the electrodes. Efficient electrical stimulation was achieved by optimizing configuration of the electrodes.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages1445-1447
Number of pages3
ISBN (Electronic)9781510897571
Publication statusPublished - 2018 Jan 1
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
Duration: 2018 Nov 112018 Nov 15

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume3

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period18/11/1118/11/15

Keywords

  • Cardiomyocyte
  • Hydrogel
  • IPSC

ASJC Scopus subject areas

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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  • Cite this

    Sumomozawa, K., Nagamine, K., Kai, H., Yoshida, S., Kaji, H., & Nishizawa, M. (2018). Hydrogel culture dish integrated with organic electrodes for efficient stimulation of hiPSC-derived cardiomyocytes. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 1445-1447). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 3). Chemical and Biological Microsystems Society.