Abstract
Polymerizable nanogels were prepared by self-assembly of cholesteryl group-bearing pullulan (CHP) with methacryloyl groups (CHPMA). The CHPMA nanogel was polymerized with 2-methacryloyloxyethyl phosphorylcholine (MPC) by radical polymerization in dilute aqueous solution. The solution properties of the polymers in water were investigated by TEM, SEC-MALS, and fluorescence quenching technique. Monodispersed hybrid nanogels of CHPMA-MPC (CM nanogels) (25-30 nm in radius of gyration) were obtained by using CHPMA nanogel as a seed-nanogel. CM nanogels have a dual cross-linking structure that is physically cross-linked with the cholesteryl groups and chemically cross-linked with the MPC polymer chains. CM nanogels trap heat-denatured carbonic anhydrase B (CAB) and prevent their aggregations. The nanogels maintained the ability of trapping and releasing enzymes by host-guest interaction of cholesteryl group and cyclodextrin.
Original language | English |
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Pages (from-to) | 710-716 |
Number of pages | 7 |
Journal | Macromolecular Bioscience |
Volume | 5 |
Issue number | 8 |
DOIs | |
Publication status | Published - 2005 Aug 12 |
Externally published | Yes |
Keywords
- Drug delivery systems
- Microgels
- Nanoparticles
- Polysaccharides
- Protein
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomaterials
- Polymers and Plastics
- Materials Chemistry