Hybrid nanogels with physical and chemical cross-linking structures as nanocarriers

Nobuyuki Morimoto, Takao Endo, Michiko Ohtomi, Yasuhiko Iwasaki, Kazunari Akiyoshi

Research output: Contribution to journalArticlepeer-review

84 Citations (Scopus)


Polymerizable nanogels were prepared by self-assembly of cholesteryl group-bearing pullulan (CHP) with methacryloyl groups (CHPMA). The CHPMA nanogel was polymerized with 2-methacryloyloxyethyl phosphorylcholine (MPC) by radical polymerization in dilute aqueous solution. The solution properties of the polymers in water were investigated by TEM, SEC-MALS, and fluorescence quenching technique. Monodispersed hybrid nanogels of CHPMA-MPC (CM nanogels) (25-30 nm in radius of gyration) were obtained by using CHPMA nanogel as a seed-nanogel. CM nanogels have a dual cross-linking structure that is physically cross-linked with the cholesteryl groups and chemically cross-linked with the MPC polymer chains. CM nanogels trap heat-denatured carbonic anhydrase B (CAB) and prevent their aggregations. The nanogels maintained the ability of trapping and releasing enzymes by host-guest interaction of cholesteryl group and cyclodextrin.

Original languageEnglish
Pages (from-to)710-716
Number of pages7
JournalMacromolecular Bioscience
Issue number8
Publication statusPublished - 2005 Aug 12
Externally publishedYes


  • Drug delivery systems
  • Microgels
  • Nanoparticles
  • Polysaccharides
  • Protein

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry


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