HVJ-E/importin-β hybrid vector for overcoming cytoplasmic and nuclear membranes as double barrier for non-viral gene delivery

Takeshi Kawazu, Hiroyuki Kanzaki, Atsushi Uno, Hideki Azuma, Takeshi Nagasaki

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    In order to enhance the nuclear import of the transgene, we prepared plasmid DNA/importin-β conjugates consisting of biotinylated poly(ethylenimine)s and recombinant streptavidin-fused importin-β. Hemagglutinating virus of Japan-envelope vector containing the PEI polyplex/importin-β conjugate showed high transfection efficiency not only in vitro but also in vivo. We showed that novel HVJ-E/importin-β-conjugated PEI polyplex hybrid vector could overcome plasma and nuclear membrane barriers to achieve effective transfection.

    Original languageEnglish
    Pages (from-to)519-524
    Number of pages6
    JournalBiomedicine and Pharmacotherapy
    Volume66
    Issue number7
    DOIs
    Publication statusPublished - 2012 Oct 1

    Keywords

    • Gene delivery
    • Hemagglutinating virus of Japan
    • Importin
    • Nuclear import
    • Poly(ethyleneimine)s

    ASJC Scopus subject areas

    • Pharmacology

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