Humoral regulation of resistin expression in 3T3-L1 and mouse adipose cells

Nobuhiro Shojima, Hideyuki Sakoda, Takehide Ogihara, Midori Fujishiro, Hideki Katagiri, Motonobu Anai, Yukiko Onishi, Hiraku Ono, Kouichi Inukai, Miho Abe, Yasushi Fukushima, Masatoshi Kikuchi, Yoshitomo Oka, Tomoichiro Asano

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167 Citations (Scopus)

Abstract

Resistin is a hormone secreted by adipocytes that acts on skeletal muscle myocytes, hepatocytes, and adipocytes themselves, reducing their sensitivity to insulin. In the present study, we investigated how the expression of resistin is affected by glucose and by mediators known to affect insulin sensitivity, including insulin, dexamethasone tumor necrosis factor-α (TNF-α), epinephrine, and somatropin. We found that resistin expression in 3T3-L1 adipocytes was significantly upregulated by high glucose concentrations and was suppressed by insulin. Dexamethasone increased expression of both resistin mRNA and protein 2.5- to 3.5-fold in 3T3-L1 adipocytes and by ∼70% in white adipose tissue from mice. In contrast, treatment with troglitazone, a thiazolidinedione antihyperglycemic agent, or TNF-α suppressed resistin expression by ∼80%. Epinephrine and somatropin were both moderately inhibitory, reducing expression of both the transcript and the protein by 30-50% in 3T3-L1 adipocytes. Taken together, these data make it clear that resistin expression is regulated by a variety of hormones and that cytokines are related to glucose metabolism. Furthermore, they suggest that these factors affect insulin sensitivity and fat tissue mass in part by altering the expression and eventual secretion of resistin from adipose cells.

Original languageEnglish
Pages (from-to)1737-1744
Number of pages8
JournalDiabetes
Volume51
Issue number6
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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  • Cite this

    Shojima, N., Sakoda, H., Ogihara, T., Fujishiro, M., Katagiri, H., Anai, M., Onishi, Y., Ono, H., Inukai, K., Abe, M., Fukushima, Y., Kikuchi, M., Oka, Y., & Asano, T. (2002). Humoral regulation of resistin expression in 3T3-L1 and mouse adipose cells. Diabetes, 51(6), 1737-1744. https://doi.org/10.2337/diabetes.51.6.1737