Humanized prion protein knock-in by Cre-induced site-specific recombination in the mouse

Tetsuyuki Kitamoto, Kenji Nakamura, Kazuki Nakao, Satoshi Shibuya, Ryong Woon Shin, Yoichi Gondo, Motoya Katsuki, Jun Tateishi

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

To establish humanized mice with a knock-in (gene replacement) technique, we constructed a targeting vector which consists of the human prion protein and the loxP sequences. The introduced human prion protein with the loxP system in the embryonic stem cells was transmitted through the mouse germ line. Transient expression of Cre recombinase in the fertilized eggs resulted in the prion protein humanized mice. The Cre-loxP-mediated gene replacement is a simple and efficient method which is generally applicable to make humanized animal models.

Original languageEnglish
Pages (from-to)742-747
Number of pages6
JournalBiochemical and biophysical research communications
Volume222
Issue number3
DOIs
Publication statusPublished - 1996 May 24

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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