TY - JOUR
T1 - Human umbilical cord-derived cells can often serve as feeder cells to maintain primate embryonic stem cells in a state capable of producing hematopoietic cells
AU - Hiroyama, Takashi
AU - Sudo, Kazuhiro
AU - Aoki, Naoko
AU - Miharada, Kenichi
AU - Danjo, Inaho
AU - Fujioka, Tsuyoshi
AU - Nagasawa, Toshiro
AU - Nakamura, Yukio
N1 - Funding Information:
We thank all members in the Cell Engineering Division for their help, discussion, and secretarial assistance. This work was supported by grants from the Ministry of Education, Culture, Sports, Science, and Technology in Japan. K.M. was supported by the Junior Research Associate grant from RIKEN.
PY - 2008/1
Y1 - 2008/1
N2 - Clinical application of human embryonic stem (ES) cells will require the establishment of methods for their culture, either in the presence or absence of human-derived feeder cells. We have tested the ability of non-immortalized cultured cells derived from human umbilical cord (HUC cells) to support ES cell culture. A primate ES cell line that had been established and maintained with mouse embryonic fibroblasts was cultured on HUC cells for >3 months (HUC-maintained ES cells). These cells retained their expression of alkaline phosphatase, SSEA-4, Oct-3/4, and to a lesser extent Nanog, but did not express Rex-1. Nevertheless, HUC-maintained ES cells could produce ectoderm-, mesoderm- and endoderm-derived cells in teratomata that they formed in immunodeficient mice. We show that HUC-maintained ES cells could give rise to hematopoietic cells, although this ability of HUC cells varied among HUC cell populations derived from different neonates. HUC cells are promising as human material with which to maintain ES cells in a state that retains their ability to produce mature cells, including hematopoietic cells.
AB - Clinical application of human embryonic stem (ES) cells will require the establishment of methods for their culture, either in the presence or absence of human-derived feeder cells. We have tested the ability of non-immortalized cultured cells derived from human umbilical cord (HUC cells) to support ES cell culture. A primate ES cell line that had been established and maintained with mouse embryonic fibroblasts was cultured on HUC cells for >3 months (HUC-maintained ES cells). These cells retained their expression of alkaline phosphatase, SSEA-4, Oct-3/4, and to a lesser extent Nanog, but did not express Rex-1. Nevertheless, HUC-maintained ES cells could produce ectoderm-, mesoderm- and endoderm-derived cells in teratomata that they formed in immunodeficient mice. We show that HUC-maintained ES cells could give rise to hematopoietic cells, although this ability of HUC cells varied among HUC cell populations derived from different neonates. HUC cells are promising as human material with which to maintain ES cells in a state that retains their ability to produce mature cells, including hematopoietic cells.
KW - ES cells
KW - Feeder cells
KW - Hematopoiesis
KW - Umbilical cord
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U2 - 10.1016/j.cellbi.2007.08.001
DO - 10.1016/j.cellbi.2007.08.001
M3 - Article
C2 - 17890111
AN - SCOPUS:39049166454
VL - 32
SP - 1
EP - 7
JO - Cell Biology International Reports
JF - Cell Biology International Reports
SN - 1065-6995
IS - 1
ER -