Human mesenchymal stem cells in rodent whole-embryo culture are reprogrammed to contribute to kidney tissues

Takashi Yokoo, Toya Ohashi, Song Shen Jin, Ken Sakurai, Yoichi Miyazaki, Yasunori Ulsunomiya, Masanori Takahashi, Yoshio Terada, Yoshikatsu Eto, Tetsuya Kawamura, Noriko Osumi, Tatsuo Hosoya

Research output: Contribution to journalArticle

173 Citations (Scopus)

Abstract

The use of stem cells has enabled the successful generation of simple organs. However, anatomically complicated organs such as the kidney have proven more refractory to stem-cell-based regenerative techniques. Given the limits of allogenic organ transplantation, an ultimate therapeutic solution is to establish self-organs from autologous stem cells and transplant them as syngrafts back into donor patients. To this end, we have striven to establish an in vitro organ factory to build up complex organ structures from autologous adult stem cells by using the kidney as a target organ. Cultivation of human mesenchymal stem cells in growing rodent embryos enables their differentiation within a spatially and temporally appropriate developmental milieu, facilitating the first step of naphrogenesis. We show that a combination of whole-embryo culture, followed by organ culture, encourages exogenous human mesenchymal stem cells to differentiate and contribute to functional complex structures of the new kidney.

Original languageEnglish
Pages (from-to)3296-3300
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number9
DOIs
Publication statusPublished - 2005 Mar 1

Keywords

  • Organogenesis
  • Regeneration

ASJC Scopus subject areas

  • General

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    Yokoo, T., Ohashi, T., Jin, S. S., Sakurai, K., Miyazaki, Y., Ulsunomiya, Y., Takahashi, M., Terada, Y., Eto, Y., Kawamura, T., Osumi, N., & Hosoya, T. (2005). Human mesenchymal stem cells in rodent whole-embryo culture are reprogrammed to contribute to kidney tissues. Proceedings of the National Academy of Sciences of the United States of America, 102(9), 3296-3300. https://doi.org/10.1073/pnas.0406878102