Human liver type pyruvate kinase: cDNA cloning and chromosomal assignment

Kenzaburo Tani, Hisaichi Fujii, Hisashi Tsutsumi, Jun Sukegawa, Kumao Toyoshima, Michihiro C. Yoshida, Tamio Noguchi, Takehiko Tanaka, Shiro Miwa

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Pyruvate kinase(PK) has four isozymes (L,R.M1,M2) that are encoded mainly by two different genes. We isolated a cDNA clone from a japanese adult liver λgt10 cDNA library by using a rat liver(L)-type PK cDNA probe. One positively hybridizing clone, hlPK-1, which contained a 1,049-base pair cDNA inset, was subjected to DNA sequence analysis. Comparisons of the sequence data with the rat PK cDNAs indicated that the cDNA encoded information for the carboxyl terminal 105 amino acids of a human L-type PK and a 3′untranslated region of 734 nucleotides. Furthermore, the karyotype analysis of several human-mouse hybrid cells and Southern blot analysis of DNAs of the hybrids with a hlPK-1 indicated that the human L-type PK gene is located on chromosome 1.

Original languageEnglish
Pages (from-to)431-438
Number of pages8
JournalBiochemical and biophysical research communications
Volume143
Issue number2
DOIs
Publication statusPublished - 1987 Mar 13

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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