TY - JOUR
T1 - Human liver type pyruvate kinase
T2 - cDNA cloning and chromosomal assignment
AU - Tani, Kenzaburo
AU - Fujii, Hisaichi
AU - Tsutsumi, Hisashi
AU - Sukegawa, Jun
AU - Toyoshima, Kumao
AU - Yoshida, Michihiro C.
AU - Noguchi, Tamio
AU - Tanaka, Takehiko
AU - Miwa, Shiro
N1 - Funding Information:
We wish to thank Dr. Akira Yoshida Genetics, Beckman Research Institute of City and Dr. Hisakazu Degawa of the Mitsui Memorial was supported by the grants from the Ministry Japan, Ministry of Education, Science and Culture Foundation for Research on Metabolic Disorders.
PY - 1987/3/13
Y1 - 1987/3/13
N2 - Pyruvate kinase(PK) has four isozymes (L,R.M1,M2) that are encoded mainly by two different genes. We isolated a cDNA clone from a japanese adult liver λgt10 cDNA library by using a rat liver(L)-type PK cDNA probe. One positively hybridizing clone, hlPK-1, which contained a 1,049-base pair cDNA inset, was subjected to DNA sequence analysis. Comparisons of the sequence data with the rat PK cDNAs indicated that the cDNA encoded information for the carboxyl terminal 105 amino acids of a human L-type PK and a 3′untranslated region of 734 nucleotides. Furthermore, the karyotype analysis of several human-mouse hybrid cells and Southern blot analysis of DNAs of the hybrids with a hlPK-1 indicated that the human L-type PK gene is located on chromosome 1.
AB - Pyruvate kinase(PK) has four isozymes (L,R.M1,M2) that are encoded mainly by two different genes. We isolated a cDNA clone from a japanese adult liver λgt10 cDNA library by using a rat liver(L)-type PK cDNA probe. One positively hybridizing clone, hlPK-1, which contained a 1,049-base pair cDNA inset, was subjected to DNA sequence analysis. Comparisons of the sequence data with the rat PK cDNAs indicated that the cDNA encoded information for the carboxyl terminal 105 amino acids of a human L-type PK and a 3′untranslated region of 734 nucleotides. Furthermore, the karyotype analysis of several human-mouse hybrid cells and Southern blot analysis of DNAs of the hybrids with a hlPK-1 indicated that the human L-type PK gene is located on chromosome 1.
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U2 - 10.1016/0006-291X(87)91372-6
DO - 10.1016/0006-291X(87)91372-6
M3 - Article
C2 - 3566732
AN - SCOPUS:0023137048
VL - 143
SP - 431
EP - 438
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -