Human liver type pyruvate kinase: cDNA cloning and chromosomal assignment

Kenzaburo Tani; Hisaichi Fujii; Hisashi Tsutsumi; Jun Sukegawa; Kumao Toyoshima; Michihiro C. Yoshida; Tamio Noguchi; Takehiko Tanaka; Shiro Miwa

doi:10.1016/0006-291X(87)91372-6

Human liver type pyruvate kinase: cDNA cloning and chromosomal assignment

Kenzaburo Tani, Hisaichi Fujii, Hisashi Tsutsumi, Jun Sukegawa, Kumao Toyoshima, Michihiro C. Yoshida, Tamio Noguchi, Takehiko Tanaka, Shiro Miwa

MED - Medical Sciences

Research output: Contribution to journal › Article

24 Citations (Scopus)

Abstract

Pyruvate kinase(PK) has four isozymes (L,R.M₁,M₂) that are encoded mainly by two different genes. We isolated a cDNA clone from a japanese adult liver λgt10 cDNA library by using a rat liver(L)-type PK cDNA probe. One positively hybridizing clone, hlPK-1, which contained a 1,049-base pair cDNA inset, was subjected to DNA sequence analysis. Comparisons of the sequence data with the rat PK cDNAs indicated that the cDNA encoded information for the carboxyl terminal 105 amino acids of a human L-type PK and a 3′untranslated region of 734 nucleotides. Furthermore, the karyotype analysis of several human-mouse hybrid cells and Southern blot analysis of DNAs of the hybrids with a hlPK-1 indicated that the human L-type PK gene is located on chromosome 1.

Original language	English
Pages (from-to)	431-438
Number of pages	8
Journal	Biochemical and biophysical research communications
Volume	143
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(87)91372-6
Publication status	Published - 1987 Mar 13

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Human liver type pyruvate kinase : cDNA cloning and chromosomal assignment. / Tani, Kenzaburo; Fujii, Hisaichi; Tsutsumi, Hisashi; Sukegawa, Jun; Toyoshima, Kumao; Yoshida, Michihiro C.; Noguchi, Tamio; Tanaka, Takehiko; Miwa, Shiro.

In: Biochemical and biophysical research communications, Vol. 143, No. 2, 13.03.1987, p. 431-438.

Research output: Contribution to journal › Article

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abstract = "Pyruvate kinase(PK) has four isozymes (L,R.M1,M2) that are encoded mainly by two different genes. We isolated a cDNA clone from a japanese adult liver λgt10 cDNA library by using a rat liver(L)-type PK cDNA probe. One positively hybridizing clone, hlPK-1, which contained a 1,049-base pair cDNA inset, was subjected to DNA sequence analysis. Comparisons of the sequence data with the rat PK cDNAs indicated that the cDNA encoded information for the carboxyl terminal 105 amino acids of a human L-type PK and a 3′untranslated region of 734 nucleotides. Furthermore, the karyotype analysis of several human-mouse hybrid cells and Southern blot analysis of DNAs of the hybrids with a hlPK-1 indicated that the human L-type PK gene is located on chromosome 1.",

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AU - Yoshida, Michihiro C.

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AU - Tanaka, Takehiko

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AB - Pyruvate kinase(PK) has four isozymes (L,R.M1,M2) that are encoded mainly by two different genes. We isolated a cDNA clone from a japanese adult liver λgt10 cDNA library by using a rat liver(L)-type PK cDNA probe. One positively hybridizing clone, hlPK-1, which contained a 1,049-base pair cDNA inset, was subjected to DNA sequence analysis. Comparisons of the sequence data with the rat PK cDNAs indicated that the cDNA encoded information for the carboxyl terminal 105 amino acids of a human L-type PK and a 3′untranslated region of 734 nucleotides. Furthermore, the karyotype analysis of several human-mouse hybrid cells and Southern blot analysis of DNAs of the hybrids with a hlPK-1 indicated that the human L-type PK gene is located on chromosome 1.

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