Human GLUT-2 overexpression does not affect glucose-stimulated insulin secretion in MIN6 cells

H. Ishihara, T. Asano, K. Tsukuda, H. Katagiri, K. Inukai, M. Anai, Y. Yazaki, J. I. Miyazaki, M. Kikuchi, Y. Oka

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9 Citations (Scopus)

Abstract

Accumulated evidence suggests that GLUT-2, in addition to its role in glucose transport, may also have other functions in glucose-stimulated insulin secretion. As a first step in addressing this possibility, we have engineered MIN6 cells overexpressing human GLUT-2 by transfection with human GLUT-2 cDNA. Stable transformants harboring human GLUT-2 cDNA exhibited an approximately twofold increase in 3-O-methyl-D-glucose uptake at 0.5 and 15 mM. Glucokinase activity or glucose utilization measured by conversion of [5- 3H]glucose to [3H]H2O was not, however, altered in the MIN6 cells overexpressing human GLUT-2. Furthermore, glucose-stimulated insulin secretion was not affected by overexpression of human GLUT-2. An abundance of GLUT-2, therefore, does not correlate with the glucose responsiveness of cells in which glycolysis is regulated at the glucose phosphorylating step. These data suggest that GLUT-2 by itself does not have significant functions other than its role in glucose transport in glucose sensing by MIN6 cells.

Original languageEnglish
Pages (from-to)E897-E902
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume269
Issue number5 32-5
DOIs
Publication statusPublished - 1995 Jan 1
Externally publishedYes

Keywords

  • glucose metabolism
  • glucose sensing
  • pancreatic β-cells
  • β-cell lines

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

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