Human geranylgeranyl diphosphate synthase: cDNA cloning and expression

Tsuyoshi Kuzuguchi, Yuiko Morita, Ikuko Sagami, Hiroshi Sagami, Kyozo Ogura

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Geranylgeranyl diphosphate (GGPP) synthase (GGPPSase) catalyzes the synthesis of GGPP, which is an important molecule responsible for the C20- prenylated protein biosynthesis and for the regulation of a nuclear hormone receptor (LXR·RXR). The human GGPPSase cDNA encodes a protein of 300 amino acids which shows 16% sequence identity with the known human farnesyl diphosphate (FPP) synthase (FPPSase). The GGPPSase expressed in Escherichia coli catalyzes the GGPP formation (240 nmol/min/mg) from FPP and isopentenyl diphosphate. The human GGPPSase behaves as an oligomeric molecule with 280 kDa on a gel filtration column and cross-reacts with an antibody directed against bovine brain GGPPSase, which differs immunochemically from bovine brain FPPSase. Northern blot analysis indicates the presence of two forms of the mRNA.

Original languageEnglish
Pages (from-to)5888-5894
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number9
DOIs
Publication statusPublished - 1999 Feb 26

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Human geranylgeranyl diphosphate synthase: cDNA cloning and expression'. Together they form a unique fingerprint.

Cite this