TY - JOUR
T1 - Human gene encoding CD38 (ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase)
T2 - Organization, nucleotide sequence and alternative splicing
AU - Nata, Koji
AU - Takamura, Toshinari
AU - Karasawa, Tadahiro
AU - Kumagai, Tomoko
AU - Hashioka, Wataru
AU - Tohgo, Akira
AU - Yonekura, Hideto
AU - Takasawa, Shin
AU - Nakamura, Shinichi
AU - Okamoto, Hiroshi
N1 - Funding Information:
We are grateful to Dr. Hideaki Kikuchi for the human genomic DNA library; Dr. Takako Sugimoto, Dr. Akira Takayama, Mrs. Takako Akiyama, Miss Nagisa Hirota, and Mr. Kei Nakagawa for their kind help; Mr. Hideto Kumagai for technical assistance; and Mr. Brent Bell for critical reading of the manuscript. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture, Japan and Japan Diabetes Foundation. T.T. and A.T. are recipients of fellowship from the Japan Society for Promotion of Science.
PY - 1997/2/28
Y1 - 1997/2/28
N2 - We have recently demonstrated that cyclic ADP-ribose (cADPR) serves as a second messenger for glucose-induced insulin secretion and that CD38 has both ADP-ribosyl cyclase (ADRC) and cADPR hydrolase activities. In this study, we determined the structure of the human CD38 gene, and showed that two mRNA forms originated by alternative splicing from the CD38 gene. The human CD38 gene consists of 8 exons that extend more than 77 kb on the human genome. Exon 1 encoded the 5'-untranslated region of the mRNA, the N-terminal end of CD38 and the putative transmembrane domain, and exon 2-8 encoded the remainder of CD38: the exon-intron organization of the human CD38 gene is similar to that of the Aplysia ADRC gene. This structural conservation between human and Aplysia genes suggests that both genes may have evolved from a common ancestral gene.
AB - We have recently demonstrated that cyclic ADP-ribose (cADPR) serves as a second messenger for glucose-induced insulin secretion and that CD38 has both ADP-ribosyl cyclase (ADRC) and cADPR hydrolase activities. In this study, we determined the structure of the human CD38 gene, and showed that two mRNA forms originated by alternative splicing from the CD38 gene. The human CD38 gene consists of 8 exons that extend more than 77 kb on the human genome. Exon 1 encoded the 5'-untranslated region of the mRNA, the N-terminal end of CD38 and the putative transmembrane domain, and exon 2-8 encoded the remainder of CD38: the exon-intron organization of the human CD38 gene is similar to that of the Aplysia ADRC gene. This structural conservation between human and Aplysia genes suggests that both genes may have evolved from a common ancestral gene.
KW - Cyclic ADP-ribose
KW - Exon
KW - Intron
KW - Multiple transcription start points
KW - RT-PCR
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U2 - 10.1016/S0378-1119(96)00723-8
DO - 10.1016/S0378-1119(96)00723-8
M3 - Article
C2 - 9074508
AN - SCOPUS:18844471424
VL - 186
SP - 285
EP - 292
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -