Human GATA-3 trans-activation, DNA-binding, and nuclear localization activities are organized into distinct structural domains

Zhuoying Yang, Lin Gu, Paul Henri Romeo, Dominique Bories, Hozumi Motohashi, Masayuki Yamamoto, James Douglas Engel

Research output: Contribution to journalArticlepeer-review

96 Citations (Scopus)

Abstract

GATA-3 is a zinc finger transcription factor which is expressed in a highly restricted and strongly conserved tissue distribution pattern in vertebrate organisms, specifically, in a subset of hematopoietic cells, in cells within the central and peripheral nervous systems, in the kidney, and in placental trophoblasts. Tissue-specific cellular genes regulated by GATA- 3 have been identified in T lymphocytes and the placenta, while GATA-3- regulated genes in the nervous system and kidney have not yet been defined. We prepared monoclonal antibodies with which we could dissect the biochemical and functional properties of human GATA-3. The results of these experiments show some anticipated phenotypes, for example, the definition of discrete domains required for specific DNA-binding site recognition (amino acids 303 to 348) and trans activation (amino acids 30 to 74). The signaling sequence for nuclear localization of human GATA-3 is a property conferred by sequences within and surrounding the amino finger (amino acids 249 to 311) of the protein, thereby assigning a function to this domain and thus explaining the curious observation that this zinc finger is dispensable for DNA binding by the GATA family of transcription factors.

Original languageEnglish
Pages (from-to)2201-2212
Number of pages12
JournalMolecular and cellular biology
Volume14
Issue number3
DOIs
Publication statusPublished - 1994 Mar

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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