Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor

Tomokazu Fukuda, Kouhei Takahashi, Shin Takase, Ai Orimoto, Takahiro Eitsuka, Kiyotaka Nakagawa, Tohru Kiyono

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Androgenetic alopecia (AGA) is the most common type of hair loss, and is mainly caused by the biological effects of testosterone on dermal papilla cells (DPCs). In vitro culturing of DPCs might be a useful tool for the screening of target molecule of AGA. However, primary DPCs cannot continuously proliferate owing to cellular senescence and cell culture stress. In this study, we introduced mutant cyclin-dependent kinase 4 (CDK4), Cyclin D1, and telomerase reverse transcriptase (TERT) into DPCs. We confirmed protein expression of CDK4 and Cyclin D1, and enzymatic activity of TERT. Furthermore, we found the established cell line was free from cellular senescence. We also introduced the androgen receptor gene using a recombinant retrovirus, to compensate the transcriptional suppressed endogenous androgen receptor in the process of cell proliferation. Furthermore, we detected the efficient nuclear translocation of androgen receptor into the nucleus after the treatment of dihydrotestosterone, indicating the functionality of our introduced receptor. Our established cell line is a useful tool to identify the downstream signaling pathway, which activated by the testosterone.

Original languageEnglish
Article number157
JournalFrontiers in Cell and Developmental Biology
Volume8
DOIs
Publication statusPublished - 2020 Mar 18

Keywords

  • androgen receptor
  • dermal papilla cells
  • dihydrotestosterone
  • immortalization
  • nuclear localization

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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