Human-derived corneal epithelial cells expressing cell cycle regulators as a new resource for in vitro ocular toxicity testing

Tomokazu Fukuda, Ryo Gouko, Takahiro Eitsuka, Ryusei Suzuki, Kohei Takahashi, Kiyotaka Nakagawa, Eriko Sugano, Hiroshi Tomita, Tohru Kiyono

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    The Draize test has been used on rabbits since the 1960s to evaluate the irritation caused by commercial chemicals in products such as cosmetics or hairdressing products. However, since 2003, such tests, including the Draize test for cosmetics, have been prohibited in European countries because they are considered problematic to animal welfare. For this reason, replacement of in vivo methods with the alternative in vitro methods has become an important goal. In this study, we established a corneal epithelial cell line co-expressing a mutant cyclin-dependent kinase 4 (CDK4), Cyclin D1, and telomerase reverse transcriptase (TERT). The established cell line maintained its original morphology and had an enhanced proliferation rate. Furthermore, the cells showed a significant, dose-dependent decrease in viability in an irritation test using glycolic acid and Benzalkonium chloride. These cells can now be shared with toxicology scientists and should contribute to increasing the reproducibility of chemical testing in vitro.

    Original languageEnglish
    Article number587
    JournalFrontiers in Genetics
    Volume10
    Issue numberJUN
    DOIs
    Publication statusPublished - 2019

    Keywords

    • Cell cycle regulators
    • Corneal epithelial cells
    • Cyclin D1
    • Cyclin-dependent kinase 4
    • Immortalization
    • Telomerase reverse transcriptase

    ASJC Scopus subject areas

    • Molecular Medicine
    • Genetics
    • Genetics(clinical)

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