Human CD8+ lymphocytes stimulated in the absence of CD4+ cells enhance IgG production by antibody-secreting B cells

Tetsu Takahashi; J. Dixon Gray; David A. Horwitz

doi:10.1016/0090-1229(91)90126-U

Human CD8+ lymphocytes stimulated in the absence of CD4+ cells enhance IgG production by antibody-secreting B cells

Tetsu Takahashi, J. Dixon Gray, David A. Horwitz

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

We describe conditions where the addition of stimulated CD8+ lymphocytes from healthy donors to autologous antibody-secreting B cells significantly enhanced IgG production by these cells. In two-step experiments, purified CD8+ lymphocytes were first cocultured with irradiated allogeneic monocytes. These cells developed interleukin 2 receptors, but proliferated only minimally in the absence of CD4+ cells. When added to antibody-secreting B cells, these CD8+ lymphocytes augmented IgG production in a dose-dependent manner in comparison with control CD8+ cells. Studies with T killer cell precursors revealed an inverse correlation between augmentation of antibody synthesis and the generation of MHC class I-restricted cytotoxic activity against lymphocytes from the allogeneic donor. Evidence is presented that CD8+ CD45RA+ CD45RO- can provide B cell help, whereas the generation of CD8+ CD45RA+ CD45RO+ cells inhibits this helper activity. Our studies support the hypothesis that in chronic diseases characterized by CD4+ cell hypofunction, CD8+ cells not only fail to down-regulate antibody production, but can provide B cell help.

Original language	English
Pages (from-to)	352-365
Number of pages	14
Journal	Clinical Immunology and Immunopathology
Volume	58
Issue number	3
DOIs	https://doi.org/10.1016/0090-1229(91)90126-U
Publication status	Published - 1991 Mar
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Pathology and Forensic Medicine
Immunology

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10.1016/0090-1229(91)90126-U

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title = "Human CD8+ lymphocytes stimulated in the absence of CD4+ cells enhance IgG production by antibody-secreting B cells",

abstract = "We describe conditions where the addition of stimulated CD8+ lymphocytes from healthy donors to autologous antibody-secreting B cells significantly enhanced IgG production by these cells. In two-step experiments, purified CD8+ lymphocytes were first cocultured with irradiated allogeneic monocytes. These cells developed interleukin 2 receptors, but proliferated only minimally in the absence of CD4+ cells. When added to antibody-secreting B cells, these CD8+ lymphocytes augmented IgG production in a dose-dependent manner in comparison with control CD8+ cells. Studies with T killer cell precursors revealed an inverse correlation between augmentation of antibody synthesis and the generation of MHC class I-restricted cytotoxic activity against lymphocytes from the allogeneic donor. Evidence is presented that CD8+ CD45RA+ CD45RO- can provide B cell help, whereas the generation of CD8+ CD45RA+ CD45RO+ cells inhibits this helper activity. Our studies support the hypothesis that in chronic diseases characterized by CD4+ cell hypofunction, CD8+ cells not only fail to down-regulate antibody production, but can provide B cell help.",

author = "Tetsu Takahashi and Gray, {J. Dixon} and Horwitz, {David A.}",

note = "Funding Information: {\textquoteright} Supported by Grant AR-29846 from the National Institutes of Health, the Adult Health Section of the State of California,a nd grantsf rom Arthritis Foundation. {\textquoteright} Present address: 2nd Department of Oral and Maxillofacial Surgery, Tohoku University School of Dentistry, 4-1 Seiryo-Machi Aoba-Ku, Sendai, 980 Japan. 3 To whom correspondence should be addressed.",

year = "1991",

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doi = "10.1016/0090-1229(91)90126-U",

language = "English",

volume = "58",

pages = "352--365",

journal = "Clinical Immunology",

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T1 - Human CD8+ lymphocytes stimulated in the absence of CD4+ cells enhance IgG production by antibody-secreting B cells

AU - Takahashi, Tetsu

AU - Gray, J. Dixon

AU - Horwitz, David A.

N1 - Funding Information: ’ Supported by Grant AR-29846 from the National Institutes of Health, the Adult Health Section of the State of California,a nd grantsf rom Arthritis Foundation. ’ Present address: 2nd Department of Oral and Maxillofacial Surgery, Tohoku University School of Dentistry, 4-1 Seiryo-Machi Aoba-Ku, Sendai, 980 Japan. 3 To whom correspondence should be addressed.

PY - 1991/3

Y1 - 1991/3

N2 - We describe conditions where the addition of stimulated CD8+ lymphocytes from healthy donors to autologous antibody-secreting B cells significantly enhanced IgG production by these cells. In two-step experiments, purified CD8+ lymphocytes were first cocultured with irradiated allogeneic monocytes. These cells developed interleukin 2 receptors, but proliferated only minimally in the absence of CD4+ cells. When added to antibody-secreting B cells, these CD8+ lymphocytes augmented IgG production in a dose-dependent manner in comparison with control CD8+ cells. Studies with T killer cell precursors revealed an inverse correlation between augmentation of antibody synthesis and the generation of MHC class I-restricted cytotoxic activity against lymphocytes from the allogeneic donor. Evidence is presented that CD8+ CD45RA+ CD45RO- can provide B cell help, whereas the generation of CD8+ CD45RA+ CD45RO+ cells inhibits this helper activity. Our studies support the hypothesis that in chronic diseases characterized by CD4+ cell hypofunction, CD8+ cells not only fail to down-regulate antibody production, but can provide B cell help.

AB - We describe conditions where the addition of stimulated CD8+ lymphocytes from healthy donors to autologous antibody-secreting B cells significantly enhanced IgG production by these cells. In two-step experiments, purified CD8+ lymphocytes were first cocultured with irradiated allogeneic monocytes. These cells developed interleukin 2 receptors, but proliferated only minimally in the absence of CD4+ cells. When added to antibody-secreting B cells, these CD8+ lymphocytes augmented IgG production in a dose-dependent manner in comparison with control CD8+ cells. Studies with T killer cell precursors revealed an inverse correlation between augmentation of antibody synthesis and the generation of MHC class I-restricted cytotoxic activity against lymphocytes from the allogeneic donor. Evidence is presented that CD8+ CD45RA+ CD45RO- can provide B cell help, whereas the generation of CD8+ CD45RA+ CD45RO+ cells inhibits this helper activity. Our studies support the hypothesis that in chronic diseases characterized by CD4+ cell hypofunction, CD8+ cells not only fail to down-regulate antibody production, but can provide B cell help.

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Human CD8+ lymphocytes stimulated in the absence of CD4+ cells enhance IgG production by antibody-secreting B cells

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