TY - JOUR
T1 - Human CD43+ B cells are closely related not only to memory B cells phenotypically but also to plasmablasts developmentally in healthy individuals
AU - Inui, Masanori
AU - Hirota, Saeko
AU - Hirano, Kumiko
AU - Fujii, Hiroshi
AU - Sugahara-Tobinai, Akiko
AU - Ishii, Tomonori
AU - Harigae, Hideo
AU - Takai, Toshiyuki
N1 - Publisher Copyright:
© The Japanese Society for Immunology. 2015. All rights reserved.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - CD20+CD27+CD43+ B (CD43+ B) cells have been newly defined among PBMCs and proposed to be human B1 cells. However, it is controversial as to whether they are orthologs of murine B1 cells and how they are related to other B-cell populations, particularly CD20+CD27+CD43- memory B cells and CD20lowCD27highCD43high plasmablasts. Our objective is to identify phenotypically the position of CD43+ B cells among peripheral B-lineage cell compartments in healthy donors, with reference to B-cell subsets from patients with systemic lupus erythematosus (SLE). We found that CD43+ B cells among PBMCs from healthy subjects were indistinguishable phenotypically from memory B cells in terms of surface markers, and spontaneous in vitro Ig and IL-10 secretion capability, but quite different from plasmablasts. However, a moderate correlation was found in the frequency of CD43+ B cells with that of plasmablasts in healthy donors but not in SLE patients. An in vitro differentiation experiment indicated that CD43+ B cells give rise to plasmablasts more efficiently than do memory B cells, suggesting that they are more closely related to plasmablasts developmentally than are memory B cells, which is also supported by quantitative PCR analysis of mRNA expression of B-cell and plasma cell signature genes. Thus, we conclude that, in healthy individuals, CD43+ B cells are closely related not only to memory B cells phenotypically but also to plasmablasts developmentally, although the developmental origin of CD43+ B cells is not necessarily the same as that of plasmablasts.
AB - CD20+CD27+CD43+ B (CD43+ B) cells have been newly defined among PBMCs and proposed to be human B1 cells. However, it is controversial as to whether they are orthologs of murine B1 cells and how they are related to other B-cell populations, particularly CD20+CD27+CD43- memory B cells and CD20lowCD27highCD43high plasmablasts. Our objective is to identify phenotypically the position of CD43+ B cells among peripheral B-lineage cell compartments in healthy donors, with reference to B-cell subsets from patients with systemic lupus erythematosus (SLE). We found that CD43+ B cells among PBMCs from healthy subjects were indistinguishable phenotypically from memory B cells in terms of surface markers, and spontaneous in vitro Ig and IL-10 secretion capability, but quite different from plasmablasts. However, a moderate correlation was found in the frequency of CD43+ B cells with that of plasmablasts in healthy donors but not in SLE patients. An in vitro differentiation experiment indicated that CD43+ B cells give rise to plasmablasts more efficiently than do memory B cells, suggesting that they are more closely related to plasmablasts developmentally than are memory B cells, which is also supported by quantitative PCR analysis of mRNA expression of B-cell and plasma cell signature genes. Thus, we conclude that, in healthy individuals, CD43+ B cells are closely related not only to memory B cells phenotypically but also to plasmablasts developmentally, although the developmental origin of CD43+ B cells is not necessarily the same as that of plasmablasts.
KW - Auto-antibody
KW - ILT3/LILRB4
KW - Natural antibody
KW - Plasmablast/plasma cell
UR - http://www.scopus.com/inward/record.url?scp=84936775550&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84936775550&partnerID=8YFLogxK
U2 - 10.1093/intimm/dxv009
DO - 10.1093/intimm/dxv009
M3 - Article
C2 - 25744616
AN - SCOPUS:84936775550
VL - 27
SP - 345
EP - 355
JO - International Immunology
JF - International Immunology
SN - 0953-8178
IS - 7
ER -