Human brain natriuretic peptide-like immunoreactivity in human brain

Kazuhiro Takahashi, Kazuhito Totsune, Masahiko Sone, Makoto Ohneda, Osamu Murakami, Keiichi Itoi, Toraichi Mouri

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95 Citations (Scopus)

Abstract

The presence of immunoreactive human brain natriuretic peptide in the human brain was studied with a specific radioimmunoassay for human brain natriuretic peptide-32. This assay showed no significant cross-reaction with human alpha atrial natriuretic peptide, porcine brain natriuretic peptide or rat brain natriuretic peptide. Immunoreactive human brain natriuretic peptide was found in all 5 regions of human brain examined (cerebral cortex, thalamus, cerebellum, pons and hypothalamus) (0.6-6.7 pmol/g wet weight, n = 3). These values were comparable to the concentrations of immunoreactive alpha atrial natriuretic peptide in human brain (0.5-10.1 pmol/g wet weight). However, Sephadex G-50 column chromatography showed that the immunoreactive human brain natriuretic peptide in the human brain eluted earlier than synthetic human brain natriuretic peptide-32. These findings suggest that human brain natriuretic peptide is present in the human brain mainly as larger molecular weight forms.

Original languageEnglish
Pages (from-to)121-123
Number of pages3
JournalPeptides
Volume13
Issue number1
DOIs
Publication statusPublished - 1992 Jan 1

Keywords

  • Atrial natriuretic peptide
  • Brain natriuretic peptide
  • Chromatography
  • Human brain
  • Radioimmunoassay

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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  • Cite this

    Takahashi, K., Totsune, K., Sone, M., Ohneda, M., Murakami, O., Itoi, K., & Mouri, T. (1992). Human brain natriuretic peptide-like immunoreactivity in human brain. Peptides, 13(1), 121-123. https://doi.org/10.1016/0196-9781(92)90149-W