Human BAC contig covering the deleted region in pancreatic cancer at 12q21

Emile M. Youssef, Kenzo Kaneko, Toshimasa Yatsuoka, Yutaka Hayashi, Masato Hoshi, Akira Horii, Toru Furukawa

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

In sporadic human primary pancreatic cancer tissues, loss of heterozygosity is frequently observed in the 1-cM region between D12S81 and D12S1719 at 12q21. Loss of this chromosome arm is known to be associate with a poor prognosis in pancreatic cancer patients. Herein we report a complete contig of human bacterial artificial chromosome (BAC) clones covering the deleted region. The region was covered by 21 BAC clones in a minimum tiling path. The clones were confirmed to exist at 12q21 by fluorescence In situ hybridization. We identified novel 40 sequence tagged sites and mapped 10 expressed sequence tags in this region. The BAC contig reported here provides an avenue for determining the complete nucleotide sequence and mining putative tumor suppressor genes in the deleted region of pancreatic cancer at 12q21.

Original languageEnglish
Pages (from-to)541-546
Number of pages6
JournalMitochondrial DNA
Volume11
Issue number6
DOIs
Publication statusPublished - 2001

Keywords

  • 12q21
  • bacterial artificial chromosome
  • expressed sequence tag
  • pancreatic cancer
  • physical map
  • sequence tagged site

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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