Human anti-gold antibodies: Biofunctionalization of gold nanoparticles and surfaces with anti-gold antibodies

Hideki Watanabe, Takeshi Nakanishi, Mitsuo Umetsu, Izumi Kumagai

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

The interface molecules designed to exhibit molecular recognitions between different species have become attractive tools for the bottom-up fabrication and hybridization of nanostructured units. Here, we focus on antibodies with high binding ability and specificity to construct a novel biomolecule interface for recognizing an inorganic material. Careful selection from a phage-displayed library of variable region heavy and light Fv chains of human antibodies using enzymelinked immunosorbent assay and surface plasmon resonance assay resulted in the identification of an antibody fragment, A14P-b2, with high affinity (K D = 1.7 nM) and specificity for gold materials. Our results indicated the potential usefulness of human antibody libraries and the effectiveness of the antibody framework for recognizing bulk material surfaces. Construction of bivalent and bispecific antibodies on the A14P-b2 platform with high affinity by means of fusion technology enabled the functionalization of gold nanoparticles and allowed selective protein accumulation on gold spots patterned on a silicon substrate. This type of antibody engineering is potentially applicable to bio-inspired materials and nanobiosensing.

Original languageEnglish
Pages (from-to)36031-36038
Number of pages8
JournalJournal of Biological Chemistry
Volume283
Issue number51
DOIs
Publication statusPublished - 2008 Dec 19

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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