HSP27 favors ubiquitination and proteasomal degradation of p27 Kip1 and helps S-phase re-entry in stressed cells

Arnaud Parcellier, Mathilde Brunet, Elise Schmitt, Edwige Col, Céline Didelot, Arlette Hammann, Keiko Nakayama, Keiichi I. Nakayama, Saadi Khochbin, Eric Solary, Carmen Garrido

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91 Citations (Scopus)

Abstract

Stress-inducible HSP27 protects cells from death through various mechanisms. We have recently demonstrated that HSP27 can also enhance the degradation of some proteins through the proteasomal pathway. Here, we show that one of these proteins is the cyclin-dependent kinase (Cdk) inhibitor p27 Kip1. The ubiquitination and degradation of this protein that favors progression through the cell cycle was previously shown to involve either a Skp2-dependent mechanism, i.e., at the S-/G2-transition, or a KPC (Kip1 ubiquitination-promoting complex)-dependent mechanism, i.e., at the G 0/G1 transition. In this work, we demonstrate that, in response to serum depletion, p27Kip1 cellular content first increases then progressively decreases as cells begin to die. In this stressful condition, HSP27 favors p27Kip1 ubiquitination and degradation by the proteasome. A similar observation was made in response to stress induced by the NO donor glyceryl trinitrate (GTN). HSP27-mediated ubiquitination of p27 Kip1 does not require its phosphorylation on Thr187 or Ser-10, nor does it depend on the SCFSkp2 ubiquitin ligase E3 complex. It facilitates the G1/S transition, which suggests that, in stressful conditions, HSP27 might render quiescent cells competent to re-enter the cell cycle.

Original languageEnglish
Pages (from-to)E281-E293
JournalFASEB Journal
Volume20
Issue number8
DOIs
Publication statusPublished - 2006 Jun 1

Keywords

  • Cell death
  • Cell proliferation
  • Proteasome
  • Stress proteins
  • Ubiquitin

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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    Parcellier, A., Brunet, M., Schmitt, E., Col, E., Didelot, C., Hammann, A., Nakayama, K., Nakayama, K. I., Khochbin, S., Solary, E., & Garrido, C. (2006). HSP27 favors ubiquitination and proteasomal degradation of p27 Kip1 and helps S-phase re-entry in stressed cells. FASEB Journal, 20(8), E281-E293. https://doi.org/10.1096/fj.05-4184fje