HSC90 is required for nascent hepatitis C virus core protein stability in yeast cells

Naoko Kubota, Yasutaka Inayoshi, Naoko Satoh, Takashi Fukuda, Kenta Iwai, Hiroshi Tomoda, Michinori Kohara, Kazuhiro Kataoka, Akira Shimamoto, Yasuhiro Furuichi, Akio Nomoto, Akira Naganuma, Shusuke Kuge

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Hepatitis C virus core protein (Core) contributes to HCV pathogenicity. Here, we demonstrate that Core impairs growth in budding yeast. We identify HSP90 inhibitors as compounds that reduce intracellular Core protein level and restore yeast growth. Our results suggest that HSC90 (Hsc82) may function in the protection of the nascent Core polypeptide against degradation in yeast and the C-terminal region of Core corresponding to the organelle-interaction domain was responsible for Hsc82-dependent stability. The yeast system may be utilized to select compounds that can direct the C-terminal region to reduce the stability of Core protein. Crown

Original languageEnglish
Pages (from-to)2318-2325
Number of pages8
JournalFEBS Letters
Volume586
Issue number16
DOIs
Publication statusPublished - 2012 Jul 30

Keywords

  • HSC90
  • HSP90 inhibitor
  • Hepatitis C virus (HCV) core protein
  • High-throughput screening
  • Stability nascent polypeptide
  • Yeast growth defect caused by core protein

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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