Hrp48, a Drosophila hnRNPA/B homolog, binds and regulates translation of oskar mRNA

Tamaki Yano, Sonia López de Quinto, Yasuhisa Matsui, Anna Shevchenko, Andrej Shevchenko, Anne Ephrussi

Research output: Contribution to journalArticlepeer-review

98 Citations (Scopus)

Abstract

Establishment of the Drosophila embryonic axes provides a striking example of RNA localization as an efficient mechanism for protein targeting within a cell. oskar mRNA encodes the posterior determinant and is essential for germline and abdominal development in the embryo. Tight restriction of Oskar activity to the posterior is achieved by mRNA localization-dependent translational control, whereby unlocalized mRNA is translationally repressed and repression is overcome upon mRNA localization. Here we identify the previously reported oskar RNA binding protein p50 as Hrp48, an abundant Drosophila hnRNP. Analysis of three hrp48 mutant alleles reveals that Hrp48 levels are crucial for polarization of the oocyte during mid-oogenesis. Our data also show that Hrp48, which binds to the 5′ and 3′ regions of oskar mRNA, plays an important role in restricting Oskar activity to the posterior of the oocyte, by repressing oskar mRNA translation during transport.

Original languageEnglish
Pages (from-to)637-648
Number of pages12
JournalDevelopmental cell
Volume6
Issue number5
DOIs
Publication statusPublished - 2004 May

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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