TY - JOUR
T1 - Hrp48, a Drosophila hnRNPA/B homolog, binds and regulates translation of oskar mRNA
AU - Yano, Tamaki
AU - de Quinto, Sonia López
AU - Matsui, Yasuhisa
AU - Shevchenko, Anna
AU - Shevchenko, Andrej
AU - Ephrussi, Anne
N1 - Funding Information:
We thank Don Rio for his generous gifts of antibodies and hrp48 transgenic and mutant stocks, and Daniel St Johnston for Staufen antibody. We especially thank Paolo Filardo for fly stocks and suggesting the oskar 3′UTR overexpression experiment, Mikita Suyama for help with statistical analysis, and Shoko Yoshida, Anna Cyrklaff, and Olivier Hachet for help with in situ hybridizations and with construction of stocks. We also thank Shoko Yoshida, Stefania Castagnetti, and the other members of the Ephrussi lab for helpful discussions during the course of this work. T.Y. was supported by fellowships from the TOYOBO Biotechnology Foundation and Cell Science Research Foundation and S.L.d.Q. by an EMBO long-term fellowship and a Marie Curie fellowship from the European Union.
PY - 2004/5
Y1 - 2004/5
N2 - Establishment of the Drosophila embryonic axes provides a striking example of RNA localization as an efficient mechanism for protein targeting within a cell. oskar mRNA encodes the posterior determinant and is essential for germline and abdominal development in the embryo. Tight restriction of Oskar activity to the posterior is achieved by mRNA localization-dependent translational control, whereby unlocalized mRNA is translationally repressed and repression is overcome upon mRNA localization. Here we identify the previously reported oskar RNA binding protein p50 as Hrp48, an abundant Drosophila hnRNP. Analysis of three hrp48 mutant alleles reveals that Hrp48 levels are crucial for polarization of the oocyte during mid-oogenesis. Our data also show that Hrp48, which binds to the 5′ and 3′ regions of oskar mRNA, plays an important role in restricting Oskar activity to the posterior of the oocyte, by repressing oskar mRNA translation during transport.
AB - Establishment of the Drosophila embryonic axes provides a striking example of RNA localization as an efficient mechanism for protein targeting within a cell. oskar mRNA encodes the posterior determinant and is essential for germline and abdominal development in the embryo. Tight restriction of Oskar activity to the posterior is achieved by mRNA localization-dependent translational control, whereby unlocalized mRNA is translationally repressed and repression is overcome upon mRNA localization. Here we identify the previously reported oskar RNA binding protein p50 as Hrp48, an abundant Drosophila hnRNP. Analysis of three hrp48 mutant alleles reveals that Hrp48 levels are crucial for polarization of the oocyte during mid-oogenesis. Our data also show that Hrp48, which binds to the 5′ and 3′ regions of oskar mRNA, plays an important role in restricting Oskar activity to the posterior of the oocyte, by repressing oskar mRNA translation during transport.
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U2 - 10.1016/S1534-5807(04)00132-7
DO - 10.1016/S1534-5807(04)00132-7
M3 - Article
C2 - 15130489
AN - SCOPUS:2342468664
VL - 6
SP - 637
EP - 648
JO - Developmental Cell
JF - Developmental Cell
SN - 1534-5807
IS - 5
ER -