HPLC analysis and optimization of enzymatic synthesis of 4'-O-(β-D- glucopyranosyl)-D-pantothenic acid

Maki Okada, Miho Kyoguchi, Toru Nakayama, Rie Hirota, Teruo Amachi, Takashi Ueda

Research output: Contribution to journalArticle

Abstract

We analyzed β-glucosidase-catalyzed transglucosylation to D-pantothenic acid using a reversed-phase HPLC system in order to obtain 4'-O-(β-D- glucopyranosyl)-D-pantothenic acid (PaG) at a higher yield. The HPLC system was simpler and more straight-forward for the PaG analysis than the previously employed bioassay method and could also be adopted for efficient isolation of PaG. Penicillium decumbens naringinase showed the highest glucosyl transfer activity to D-pantothenic acid, and the reaction using smaller amounts of naringinase for prolonged periods of reaction time (70 h<) was important to attain higher yields of glucosyl transfer. Maximum overall yields of PaG of 10 and 4% (mol/mol, based on D-pantothenic acid) were obtained using β β'-trehalose and cellobiose, respectively, as glucosyl donors. The value was 3.6- and 1.4-times higher, respectively, than that obtained by previous synthesis and isolation procedures.

Original languageEnglish
Pages (from-to)101-104
Number of pages4
JournalJournal of nutritional science and vitaminology
Volume46
Issue number2
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Keywords

  • 4'-O-(β-D-glucopyranosyl)-D-pantothenic acid
  • D- pantothenic acid
  • Transglycosylation
  • β-glucosidase

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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