How the formation process influences the structure of BChl c aggregates

Mitsuo Umetsu, Zheng Yu Wang, Jun Zhang, Takasada Ishii, Kaku Uehara, Yoji Inoko, Masayuki Kobayashi, Tsunenori Nozawa

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The change of absorption spectra has been measured during the drying process of (31R)bacteriochlorophyll (BChl) c from diethyl ether, dichloromethane (CH2Cl2) and carbon tetrachloride (CCl4) solutions. Absorption maxima of the Q(y)(0-0) transition of BChl c appear at 659 nm in diethyl ether, 680 nm in CH2Cl2 and 710 nm in CCl4. All these peaks are red-shifted to about 740 nm with formation of solid high aggregates when the solutions are completely dried. Fourier transform infrared spectra of the three solid aggregates are almost identical. However, magnetic circular dichroism and circular dichroism spectra are different and can be explained in terms of variations in stacking size of the aggregates and molecular arrangement of BChl c. Small-angle X-ray diffraction has been observed only for the aggregates treated with CH2Cl2, and the same sample gave rise to highly resolved cross polarization/magic angle spinning 13C nuclear magnetic resonance spectrum. The results suggest that molecular ordering of the solid-state BChl c aggregates is highly dependent on the formation process which is largely determined by the solvent used.

Original languageEnglish
Pages (from-to)229-239
Number of pages11
JournalPhotosynthesis Research
Volume60
Issue number2-3
DOIs
Publication statusPublished - 1999 Jan 1

Keywords

  • BChl c-stacking size
  • Chlorosomes
  • High aggregates
  • Ordering of BChl c molecules
  • Time variation of absorption spectra
  • X-ray diffraction

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

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  • Cite this

    Umetsu, M., Wang, Z. Y., Zhang, J., Ishii, T., Uehara, K., Inoko, Y., Kobayashi, M., & Nozawa, T. (1999). How the formation process influences the structure of BChl c aggregates. Photosynthesis Research, 60(2-3), 229-239. https://doi.org/10.1023/a:1006258029191