Hormone-induced apoptosis by Fas-nuclear receptor fusion proteins: Novel biological tools for controlling apoptosis in vivo

Hirohide Takebayashi, Hiroji Oida, Kazuko Fujisawa, Masahiro Yamaguchi, Takatoshi Hikida, Manabu Fukumoto, Shuh Narumiya, Akira Kakizuka

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

We have created fusion proteins between Fas and the ligand-binding domain of the estrogen or retinoic acid receptor. Murine fibrosarcoma L929 cells and human cervical carcinoma HeLa cells expressing the fusion proteins demonstrated apoptotic phenotypes in a tightly estrogen- or retinoic acid- dependent manner in vitro. Moreover, the fusion protein-expressing L929 cells transplanted into nude mice were also killed through apoptosis alter injection of an estrogen agonist. This represents a novel system, 'cell targeting,' that can eliminate cells not only in vitro but also in vivo through the activation of a natural suicide machinery, i.e., apoptosis, by currently used hormones. This system implies wide applications not only in developmental biology and neurobiology but also in medicine, especially for cancer gene therapy.

Original languageEnglish
Pages (from-to)4164-4170
Number of pages7
JournalCancer Research
Volume56
Issue number18
Publication statusPublished - 1996 Sep 15

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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