TY - JOUR
T1 - Hormone-induced apoptosis by Fas-nuclear receptor fusion proteins
T2 - Novel biological tools for controlling apoptosis in vivo
AU - Takebayashi, Hirohide
AU - Oida, Hiroji
AU - Fujisawa, Kazuko
AU - Yamaguchi, Masahiro
AU - Hikida, Takatoshi
AU - Fukumoto, Manabu
AU - Narumiya, Shuh
AU - Kakizuka, Akira
PY - 1996/9/15
Y1 - 1996/9/15
N2 - We have created fusion proteins between Fas and the ligand-binding domain of the estrogen or retinoic acid receptor. Murine fibrosarcoma L929 cells and human cervical carcinoma HeLa cells expressing the fusion proteins demonstrated apoptotic phenotypes in a tightly estrogen- or retinoic acid- dependent manner in vitro. Moreover, the fusion protein-expressing L929 cells transplanted into nude mice were also killed through apoptosis alter injection of an estrogen agonist. This represents a novel system, 'cell targeting,' that can eliminate cells not only in vitro but also in vivo through the activation of a natural suicide machinery, i.e., apoptosis, by currently used hormones. This system implies wide applications not only in developmental biology and neurobiology but also in medicine, especially for cancer gene therapy.
AB - We have created fusion proteins between Fas and the ligand-binding domain of the estrogen or retinoic acid receptor. Murine fibrosarcoma L929 cells and human cervical carcinoma HeLa cells expressing the fusion proteins demonstrated apoptotic phenotypes in a tightly estrogen- or retinoic acid- dependent manner in vitro. Moreover, the fusion protein-expressing L929 cells transplanted into nude mice were also killed through apoptosis alter injection of an estrogen agonist. This represents a novel system, 'cell targeting,' that can eliminate cells not only in vitro but also in vivo through the activation of a natural suicide machinery, i.e., apoptosis, by currently used hormones. This system implies wide applications not only in developmental biology and neurobiology but also in medicine, especially for cancer gene therapy.
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M3 - Article
C2 - 8797587
AN - SCOPUS:0029810082
VL - 56
SP - 4164
EP - 4170
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0008-5472
IS - 18
ER -