Honeycomb-like architecture produced by living bacteria, Gluconacetobacter xylinus

Yasumitsu Uraki, Junji Nemoto, Hiroyuki Otsuka, Yutaka Tamai, Junji Sugiyama, Takao Kishimoto, Makoto Ubukata, Hiroshi Yabu, Masaru Tanaka, Masatsugu Shimomura

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Bacterial cellulose (BC)-producing bacterium, Gluconacetobacter xylinus (ATCC53582), was found to move along linear microgrooves of a stripe-patterned cellulosic scaffold. On the basis of this finding, fabrication of honeycomb-patterned BC was attempted by controlling the bacterial movement using a agarose film scaffold with honeycomb-patterned grooves (concave type). The patterned agarose film was prepared by three steps. The first was transcription of a honeycomb-patterned polycaprolactone film template with polydimethyl siloxane. When the bacteria were cultured on the scaffold under atmospheric conditions, only bacterial proliferation was observed. Honeycomb-patterned BC was obtained when cultured under a humid CO2 atmosphere. Electron diffraction and polarized microscopic observation showed that the patterned BC comprised of the well defined cellulose Iα microfibrils. As another attempt to fabricate honeycomb-patterned BC, the bacteria were cultured on the patterned cellulose and agarose film with convex type of honeycomb. This culture yielded no honeycomb-patterned BC. Therefore, concave type honeycomb scaffold is more suitable to fabricate honeycomb-patterned BC.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalCarbohydrate Polymers
Volume69
Issue number1
DOIs
Publication statusPublished - 2007 May 1

Keywords

  • Cellulose Iα
  • Electron diffraction
  • Honeycomb-patterned bacterial cellulose
  • Self-organization

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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